钩藤具有熄风定惊、清热平肝的功效，是临床治疗帕金森病（PD）的重要中药。本项目组和文献前期研究均表明，钩藤能有效改善PD小鼠的行为恢复，对神经细胞和神经元有显著保护作用。但钩藤抗PD活性的药效物质及作用机制尚不明确，因此本项目以中医药理论为指导，综合运用中药化学、药理学、药物代谢及药动学等多学科交叉的研究方法，从体外到体内、从单体成分到整体成分，阐明钩藤抗PD的体内药效物质；以主要体内活性成分（包括活性代谢产物的原型成分）在体内和钩藤中的含量为活性成分定量参数，以各活性成分在不同模型下的作用强度为活性参数，建立体内成分与活性之间的“定量组-效相关谱”，评价不同成分的活性贡献，系统表征钩藤抗PD的多成分、多途径调控机制。项目的实施将为钩藤临床精准用药和创新药物发现提供科学依据，为中药多成分、多途径作用表征的方法学提供有益探索，为诠释中医治疗PD的“平肝潜阳、活络熄风“理论提供重要参考。

Uncariae Ramulus Cum Uncis is one of the famous traditional Chinese medicines (TCMs) for the clinical treatment of Parkinson's disease (PD). Preliminary results by our group and other groups showed that Uncariae Ramulus Cum Uncis effectively improved the behavior recovery of PD mice, and exhibited a significant protective effect on nerve cells and neurons. However, the pharmacologically active ingredients of Uncariae Ramulus Cum Uncis against PD and its regulatory mechanism are not clarified definitely. .In this project, we intends to clarify the in vivo bioactive constituents of Uncariae Ramulus Cum Uncis against PD from the perspectives of combinational analysis of the constituents and activity in vitro and those in vivo, and the single compound and multiple components, by using interdisciplinary research methods of Natural Medicinal Chemistry, Pharmacology, Drug Metabolism and Pharmacokinetics. Furthermore, systematical investigations on anti-Parkinson’s efficacy and molecular mechanism of the in vivo constituents will be carried out, for the purpose of establishing the "quantitative composition-activity relationship" and clarifying the regulatory mechanism of Ramulus Uncariae cum Unicisbased on multi-components and multi-pathways. .The implementation of this project will provide scientific evidences for the clinical precision use and innovative drug development based on Ramulus Uncariae cum Unicis, act as a useful strategy for the characterization of regulatory mechanism based on multi-components and multi-pathways, and also supply reference data for interpreting the scientific meaning of TCM theory of "smoothing the liver and suppressing the Yang, activating the meridians and extinguishing the wind" in the treatment of PD.