结核分枝杆菌(M.tb) 为结核病的病原菌，是典型的细胞内寄生菌。分枝杆菌感染宿主后，主要定居于巨噬细胞内。巨噬细胞是机体防御系统的第一道屏障，一方面发挥着抵抗结核分枝杆菌的作用，另一方面又是结核分枝杆菌体内滞留、造成潜伏感染的主要场所。巨噬细胞与结核分枝杆菌的相互作用对结核病的发生、发展起着非常重要的作用。本研究采用蛋白质组学iTRAQ技术，以不同毒力牛分枝杆菌(M.bovis)菌株感染后的小鼠巨噬细胞RAW264.7作为体外细胞模型，对巨噬细胞的存活、细胞的极化特点、主要细胞因子产生及其作用规律、胞内蛋白、细胞凋亡特点及信号通路变化规律等方面进行分析。本研究从细胞生物学、蛋白质组学等层面展开研究，为全面探究巨噬细胞蛋白组分的变化规律、牛分枝杆菌致病机理、巨噬细胞的抗感染机制及其凋亡规律奠定了基础。在阐释巨噬细胞胞内蛋白及细胞因子作用规律、信号通路作用机制等方面具有重要意义。

Mycobacterium tuberculosis(M.tb) is pathogenic bacteria of tuberculosis, which is a typical intracellular parasitic bacteria. M.tb usually locates in macrophage when it infect the host. And macrophage is the first barricade to body defense system.On one hand, macrophage plays an important role to defend M.tb,on the other hand , macrophage can be the main location for the persistence and potential infection of M.tb. Interaction between macrophage and M.tb acts as most important role to occurrence and development of tuberculosis. In this study, we use RAW264.7 as an vitro macrophage model stimulated by Mycobacterium bovis (M. bovis) with different virulence. And iTRAQ technology will be applied mainly to analyze macrophage survival , cellular polarization, main cytokine’s production and its effect regularity, characteristic of endocelluar protein and apoptosis of macrophage, change regularity of signal pathway. The study will establish the base to explore completely in the fields of change regularity of inner macrophage proteins components, pathogenic mechanism of M.tb, mechanism of anti-infection and apoptosis of macrophage by means of methods involved in cell biology, molecular biology, immunology, proteomics. The important significance of the study focuses on demonstrating effect regularity of macrophage inner protein and cytokines, mechanism of signal pathway of macrophage and anti-infection of M. bovis.