肌腱运动损伤作为一种常见的运动性损伤疾病目前修复困难，肌腱标志及分化调控知识缺乏严重地阻碍了干细胞肌腱分化及其应用于肌腱运动损伤的再生治疗。本研究拟在前期创新发现SCX+NES+肌腱干细胞亚群，建立肌腱组织工程模型（Stem Cells 2009），发现转录因子SCX与力学协同(Sci Rep 2012)并受MKX调控的基础上，深入探索肌腱关键转录因子SCX在SCX+NES+肌腱干细胞分化和再生中的作用及其与其他因子（MKX和力学刺激）相互调控的效应和机制。本项目运用肌腱干细胞技术、生物工程技术和分子免疫等技术在肌腱发育模型和组织工程模型上，研究SCX+NES+肌腱干细胞亚群的生物特性，探明肌腱关键转录因子SCX与MKX和力学相互调控的机制。预期本研究将1.为肌腱干细胞生物学及肌腱分化调控提供新知识；2.为促进肌腱损伤的再生治疗提供理想的种子细胞和力学康复手段 。

Tendon injury is one of the most common sports injury but current therapeutics can not be satisfied. Stem cell transplantation is very promising, however, the knowledge about the regulatory mechanism underlying tenocytes differentiation is very limited, which hinders the development of tissue engineering technology for tendon sports injury repair. Based on our previous innovation findings, including 1) SCX+NES+ tendon stem cells. 2) SCX have synegetic function with mechnical force on tendon differentitaiton (Sci Rep 2012). 3) SCX is regulated by another tendon transcription factor MKX. 4) Our novel model for constuct and evalutate engineered tendon(Stem Cells 2009). This study aims to identify the roles of SCX on the SCX+NES+ tendon stem cells differentiation and regeneration; as well as the synergistic effect and mechanism between transcription factors and mechanical force on promoting tenocytes induction and maturation. This study expect to provide 1) novel knowledge for tendon stem cells differentiation and maturation; 2) provide optimal seed cells and mechanical methods for the regeneration and Rehabilitation of tendon injury.