膝关节运动损伤十分常见且难以修复，尤其是难以再生关节内的多个硬-软组织复杂连接结构，如骨-软骨和骨-韧带等。本研究拟设计三个课题系统解析和精准再生运动系统复杂连接结构。课题一：通过单细胞分析技术，解析复杂连接结构的细胞图谱，运用类器官培养技术，筛选和探究能够再生连接结构的仿生细胞组成；课题二：通过蛋白质谱等技术解析连接结构的细胞外基质成分谱，运用计算化学设计合成界面黏合分子与胶体材料；课题三：仿生连接处的生物成分，通过“光”速生物打印组装，体内外精准生物构建复杂连接结构。本研究的开展将在“细胞-细胞、细胞-微环境”层面系统性揭示复杂结构的组织互作与再生规律，形成膝关节复杂结构再生的新思路、新材料与新技术，实现运动创伤的精准医疗。

Knee injuries are worldwide common and difficult to repair, especially for the regeneration of multiple hard-soft tissue complex junctions within the joints, such as bone-cartilage and bone-ligament connections. This study plans to design three projects to systematically analyze and precisely regenerate complex connection structures in motor system. Project 1: using single cell analysis technics to dissect the cell subpopulation map of complex connection structures, and organoid culture techniques to screen and explore the cell subtypes with regenerative ability. Project 2: using protein spectrum analysis technic to investigate the extracellular matrix map, and using computational chemistry to design and synthesize the interfacial bonding molecules and materials. Project3: imitating the biological components of the complex junctions, through the "light" speed bioprinting and integration, to achieve complex connection structure precise rebuilding in vitro and in vivo. This research will systematically reveal the knowledges of tissue interaction and regeneration of complex structures at the "cell-cell', ‘'cell-microenvironment" level, and create new ideas, new materials and new technologies for the regeneration of complex structures of the knee, so as to achieve precise regeneration for sports injuries.