Aptamer-Fe3O4@Cu/Au/Pt NPs双功能探针构建及其食源性病原菌检测与抑制性能研究

核酸适配体（Aptamer）因具有分子量小、靶标广泛、易于合成修饰、无免疫原性以及序列设计灵活且构型变化多样等优点，在食源性病原菌检测应用领域展现出其独特优势。然而，目前基于核酸适配体的细菌检测技术仍然面临着探针制备相对复杂、检测流程较繁琐、难以多目标分析以及难以实现多功能等挑战。基于此，本项目拟结合纳米技术与核酸分子探针技术，以具有磁、拟酶催化、荧光淬灭以及光热转换多种性能的Fe3O4@Cu/Au/Pt NPs为功能性载体，以发夹型核酸适配体探针为识别信号元件，通过表面自组装修饰构建Aptamer-Fe3O4@Cu/Au/Pt NPs双功能探针；分别从目标菌分析、拟酶催化与光热协同抑菌性能两个不同层面系统评估双功能探针的细菌检测与抑制效果，发展快速、简便、灵敏、特异的食源性病原菌检测新方法以及抑菌新技术。本项目的执行将为食源性病原菌所引发疾病的预防与控制提供重要支持。

Aptamer shows unique superiorities for applications in the detection field of foodborne pathogens owing to its advantages of low molecular weight, wide range of target molecules, easy chemical synthesis and labeling, low immunogenicity, flexible and diverse sequence design and so on. However, at present, the study on the detection of pathogens by aptamer is still facing with many challenges, such as relatively complex preparation of probes, relatively complicated process of detection, difficulties in multiplex analysis and construction of multifunctional probes. Thus, in this project, by combining the technologies of nano and nucleic acid molecular probe, aptamer-Fe3O4@Cu/Au/Pt NPs based dual-functional probes will be constructed by the modification of hairpin aptamer probes with recognizing signal units on the surface of Fe3O4@Cu/Au/Pt NPs with the properties of magnetism, enzyme-like catalytic activity, fluorescence quenching and photo-heat conversion. Then we will systematacially investigate the performance of the dual-functional probes for detection of pathogens and synergetic antibacterial effects of enzyme-like catalytic activity and photothermal conversion. This holds great promise to provide new ideas and methods not only for fast, facile, sensitive and specific determination of foodborne pathogens but also for the development of new antibacterial techniques. The execution of this project would provide important support for the prevention and control of diseases caused by foodborne pathogens.