忍冬属植物抗旱性综合评价及其相关MYB转录因子的克隆与鉴定

忍冬属植物是药典中金银花和山银花的来源植物，同时也是岩溶区的适生植物；MYB转录因子家族成员广泛参与植物次生代谢调控和对激素及环境因子应答；忍冬属植物抗旱综合评价及其抗旱相关的MYB转录因子克隆与功能验证对石漠化治理的引种工作具有实践意义，对于阐明植物适应岩溶环境机制具有理论意义。.本项目首先用主成分分析和隶属函数法对几种忍冬属植物进行抗旱综合评价；以干旱胁迫第一主成分因子的生理指标为依据，对其中抗性最好的种通过数字基因差异表达谱分析,结合q-PCR的方法进行响应干旱的相关MYB转录因子筛选；然后将侯选基因以农杆菌介导的方式转化到拟南芥和忍冬属植物中进行功能验证，期望筛到重要的与抗逆相关的MYB转录因子。.本项目能够为有经济价值的植物提供优良的抗逆基因，在为阐明忍冬属植物药用品质与环境之间的关系提供线索的同时也能为阐明植物对岩溶环境的适应机制提供数。

The plants of Flos Lonicerae are not only the resource plant of medical herb jinyinhua, but also are adaptable plant to Karst environment; MYB factors are important in regulatory networks controlling development, metabolism and responding to biotic and abiotic stresses. The cloning and functional verification of MYB transcription factors related to drought is important to explain the adaptation mechanism of plant to Karst environment and control rocky desertification.. In this study, the comprehensive drought resistance ability of different Flos Loniceraes. is evaluated according to their physiological indexes under drought through principal component analysis and subordinate function method. Then the specie with the highest resistance to drought is used as the material from which the gene of MYB factors is selected through digital gene expression profile of the samples under different degree of drought. The candidate genes selected are cloned and functional verification by the transgenic Arabidopsis and Flos Lonicerae.. This study can provide the resistant genes for the economic plant, and also be useful to explain the relation of medical quality and environment of Lonicerae genus and the mechanism of adaptation to Karst.