子宫内膜癌缺乏有效的腹膜后淋巴结转移评估与治疗手段，多数患者接受了不必要的淋巴结切除术。课题组针对此临床困境，基于前期工作基础，利用纳米技术制备葡萄糖、聚乙二醇和吲哚菁绿三种功能分子修饰的多功能纳米金颗粒，使其具备淋巴靶向、肿瘤靶向、荧光显像及X线显像/放疗增敏特性。课题组筛选葡萄糖受体高表达内膜癌细胞系，体外实验验证多功能纳米金颗粒的肿瘤细胞靶向、放疗增敏和荧光显像特性。建立内膜癌小鼠腹膜后淋巴结转移瘤模型，体内药物分布和药代学实验验证其淋巴靶向和肿瘤靶向性。荷瘤小鼠术前Micro-CT扫描探讨其淋巴结转移瘤显像增敏功能；术中活体荧光成像明确其病灶实时荧光显像指导手术的功能；术后X线照射淋巴结转移瘤验证其放疗增敏特性。本课题利用一个平台同时实现腹膜后淋巴结病灶的CT显像增敏、实时荧光显像和放疗增敏，实现基于精确显像基础上的精确治疗，避免系统性淋巴结切除术的弊端，有望实现肿瘤诊治一体化。

Endometrial cancer with retroperitoneal lymph node metastasis currently lack effective assessment and management methods. Most patients have to receive systemic retroperitoneal lymphadenectomy unnecessarily. Nano-targeting technology offers a fascinating diagnosis and therapeutic option in cancer due to the unique biological properties of nanoparticles. In this project we are going to prepare one kinds of multi-functional Gold Nanoparticles(mf-GNPs). Three different functional molecules would be coupled on the surface of GNPs particles, including glucose(Glu), polyethyleneglycol (PEG) and indocyanine green(ICG). These molecules could offer mf-GNPs a variety of distinctive properties such as lymphatic targeting, tumor targeting, fluorescence imaging as well as X-ray based imaging sensitization and radiotherapy enhancement. Human endometrial cancerous cell line with high specific glucose receptors expression level on the surface of cytomembrane would be identified for further studies. Cellular uptake and X-ray irradiation experiments in vitro would be performed to confirm the targeting and radiosensitization properties of mf-GNPs to tumor cells. A mice endometrial cancer retroperitoneal lymph-node metastasis animal model would be established for in vivo studies. Drug distribution and pharmacokinetic studies in animal tissues following mf-GNPs administration would be performed to confirm the lymph and tumor targeting properties of mf-GNPs in vivo. Eventually, micro-CT imaging in vivo would be used to explore the diagnostic accuracy of mf-GNPs based CT imaging for retroperitoneal lymph node metastatic lesions. In vivo bioluminescence imaging would be used to identify the metastatic lymph nodes to enable accurate surgical removal of the target lesion during operation. Metastatic lymph nodes X-ray irradiation study in vivo would be performed to confirm the radiotherapy enhancement property of mf-GNPs. In this project we integrate multiple functions into a single GNPs particle to realize the simultaneous cancer diagnosis and therapy. The established gold nanoplatform promises a powerful tool for imaging sensitization, in vivo bioluminescence imaging and radiotherapy enhancement to retroperitoneal lymph node metastatic lesions in endometrial cancer. The translation of mf-GNPs into the clinic will also have significant impact on the care of cancer patients using precise image-guided precise interventions in the near future.