基于微藻固定化培养的膜式光生物反应器内多相流动传输与代谢调控机理研究

基于光合微藻高密度固定化培养的多孔选择性渗透膜-生物膜光生物反应器是一个含气、液流动和光生化反应的的复杂能质传输反应体系。本项目以小球藻为研究对象，主要研究气、液流动条件下小球藻光合固碳过程中CO2、光能传递及与生物膜生长代谢间的耦合作用关系。课题首先开展藻细胞光生化反应的微观机理研究，获得能、质传输对光合微藻细胞生长动力学的影响规律；分别研究气液流动、光能和CO2传递对细胞运动和吸附、生物膜生长代谢的影响规律，以揭示能质传递对细胞运动、吸附与生物膜形成的耦合作用关系。在此基础上，建立渗透膜-生物膜光生物反应器内含光能和CO2传递影响的生物膜生长元胞自动机模型和成熟生物膜内含光生化反应的多元多相流体流动与传输理论模型，揭示能源微藻光自养培养系统中的能质流动特性及环境应答机制，提出基于能质耦合传递影响以强化生物膜生长及光生化转化为目的的多目标优化与代谢调控集成理论。

The permeable membrane-biofilm photo-bioreactor used for high-density cultivation of algae by immobilized-cell method is a complicated reaction system with processes of gas and liquid flow accompanying with photo-biochemical reactions. In this project, characteristics of mass and light transfer, as well as biofilm formation during carbon fixation by chlorella cells under the conditions of gas-liquid flow will be investigated. It can provide theoretical basis to the high-density cultivation of photosynthetic microalgae. Specifically, the microcosmic mechanism of photo-biochemical reaction within algae cells will be first discussed to reflect the effect of energy and mass transfer on growth kinetics of cells. Then, the effects of gas-liquid flow, light energy and CO2 transfer on movement and adsorption of chlorella cells, biofilm formation will be respectively studied in order to reveal the coupling relationship between energy - mass transfer and biofilm formation. Based on above research results, a CA model on biofilm growth and a theoretical model on energy-mass transfer and multiphase fluid flow accompanying photo-biochemical reactions will be established in the bioreactor to indicate the characteristics of flow of heat and mass, as well as response mechanism to environment of algae. Finally, a integration theory on multi-objective optimization and metabolic regulation will be obtained to intensify biofilm growth and photo-biochemical conversion of algae in view of interactions of mass transfer with light transmission.