dlo-miR167家族在龙眼体胚发生过程中的作用机制研究

microRNA167家族在植物胚胎发育中具有重要调控作用，而其在木本植物体胚中的作用还不清楚。在本项目中，利用龙眼体胚发生系统，分离pri-miR167、启动子及靶标；利用qPCR技术检测miR167家族及其靶标在不同发育阶段的表达情况及其在不同激素和胁迫处理下的胚性愈伤组织中的转录水平；进行dlo-miR167家族与关键靶标互作机制研究；并将miR167家族不同成员转化龙眼和拟南芥进行功能鉴定。以探索dlo-miR167家族及其靶标在龙眼体胚中的互作机制，为揭示龙眼体胚发生的分子机制提供新的线索，并为龙眼胚胎发育的调控提供参考。

microRNA167 family has an important regulatory role in plant embryo development, but its molecule mechanism during somatic embryogenesis in woody plants is still unclear. In this project,using the system of longan somatic embryogenesis, we plan to isolate genes of pri-miR167, its promoter and its target, to carry out the qPCR analyses between miR167 family and its target,to analyses the interaction mechanism of dlo-miR167 family and key target; and further condcut functional checking of dlo-miR167 family, different members of the conversion of longan and Arabidopsis functional identification. Finally,the results which will systematically clarify the expressional and regulatory mechanism between miR167 family and its target during longan somatic embryogenesis, and further provide scientific evidences for regulating the in vivo and in vitro embryogenesis in longan.