克隆植物草地早熟禾（Poa pratensis L.）是我国北方应用最多的优良冷季型草坪草。水分和氮素是限制其生长和观赏质量的两个极其重要因素。本项目基于前期草地早熟禾单株水分胁迫下氮代谢及其机制研究基础上，采用形态学、生理学、种群生态学及分子生物学等多学科的理论和先进技术，拟创新性地探索草地早熟禾克隆分株群体水平上响应干旱胁迫的氮素生理整合特征，揭示氮素在群体水平上克隆分株间传输、转移、分配和共享的格局及其机制。主要研究内容有：干旱胁迫下草地早熟禾克隆分株形态可塑性、生长发育与氮素生理整合；草地早熟禾响应干旱克隆分株间氮素生理整合的方向、强度及传输格局；草地早熟禾克隆分株细胞中酶系统、渗透调节物质、生理活性物质及其基因调控氮素生理整合的机制。综合分析比较筛选出缺水条件下草地早熟禾克隆分株有效氮素生理整合特征指标。研究结果对丰富植物养分代谢与传输理论及培育草坪新品种具有重要理论和现实意义。

The clone plant of Kentucky bluegrass (Poa pratensis L.) is the most widely used cool-season turfgrass in Northern China. Both water and nitrogen content are two important factors adjusting the growth and ornamental quality. The current project continues to investigate the physiological adjustment mechanism of Kentucky bluegrass to nitrogen under drought stress and to reveal the pattern and mechanism of nitrogen transmission, transfer, distribution, and sharing between clonal ramets in population, by morphological, physiological, population ecological, and molecular biological theories and advanced technologies. The project is based on the characteristics and mechanisms of nitrogen metabolism of unique Kentucky bluegrass under water stress. The main study includes: investigation of clone ramet morphological plasiticity, growth, and nitrogen physiological integration, and its morphological mechanism under drought stress; study of direction, intensity, and transmission pattern of nitrogen integration between clone ramet under drought stress; study of adjustment mechanism of nitrogen physiological integration by enzyme system, osmoregulation substances, physiologically active substances and its genes expression; screening effective indexes of nitrogen integration of Kentucky bluegrass under drought stress.The results will be important to support the theory of plant nutrition metabolism and transportation, as well as to be significant in practice for turfgrass breeding.