新疆哈萨克族食管癌发病率高（150/10万），远高于全国平均水平（15/10万），浸润转移是主要死亡原因，机理有待阐明。上皮间质转化 (EMT)在癌浸润转移中起关键作用。 我们既往以哈族食管鳞癌MAPK信号通路和微小RNA let-7调控机制为导向的研究发现：ERK信号通路的活化参与哈族食管癌的发生，与该通路相关基因 let-7表达与患者淋巴结转移相关，且在转录后水平调控HMGA2表达。基于此结果，本项目围绕哈族食管癌EMT调控与浸润转移开展研究，拟从体外（细胞为模型）、动物模型（裸鼠致瘤）和人体内（癌组织） 三个层面， 运用多种方法（RNA干扰、Transwell体外侵袭、荧光素酶活性分析等），探讨EMT过程中重要信号通路因子（TGF-β、ERK、HMGA2、let-7、Snail）调控与哈族食管鳞癌EMT恶性表型之间的关系，并为寻找基于食管癌EMT的分子预测、指导个体化治疗提供新靶点。

The incidence of esophageal squamous cell carcinoma (ESCC) in Kazakh ethnic was highest with 150/100,000, which is far higher than the national average with 15 /100,000. The major mortality cause for Kazakh ESCC (KESCC) was cancer invision and metastasis. However, the mechanism of invision and metastasis in KEC remains to be clarified..The epithelia-mesenchymal transition(EMT) played a important role in the progression of cancer invision and metastasis. In our previous study, the result showed p-ERK may play a role in the earlier pathogenesis of KESCC. Moreover, let-7 and HMGA2, as downstream gene of MAPK pathway, were involved in KESCC carcinogenesis, and HMGA2 could be negatively regulated by let-7 at the post-transcriptional level in KESCC.There was a correlation between let-7 lower expression and lymph node metastasis in KESCC patients. .In present project, based on several research platforms, such as RNA interference,Transwell cell migration assay, Luciferase reporter assay and etc, the regulation mechanism between the variation(TGF-β、MEK/ERK、let-7、HMGA2、Snail) in EMT signal pathway of KESCC and characteristics of the pathological maligancy(invision ,lymph node metastasis) was explored both ESCC cells,animal model and tissue level..The present contribution will provide not only regulation mechanism of EMT in KESCC, but also the potential targets for molecular classification of EMT in KESCC, which could be beneficial for prediction, prognosis and individual treatment for KESCC.