解析胆囊癌发生转移的分子机制是目前肿瘤研究的重点。课题组认识到编码基因的mRNA可以通过竞争非编码小RNA（miRNA）的方式调控其他功能性mRNA，构成功能相似，彼此调控的竞争性内源RNA（ceRNA）分子。前期工作中，我们证实睾丸蛋白聚糖1（SPOCK1）是影响胆囊癌转移过程的关键分子，可促进胆囊癌细胞侵袭转移和EMT转化。通过生物信息学预测与芯片分析，我们发现同属于SPARC家族的TESTICAN-2是SPOCK1的ceRNA。并发现SPOCK1和TESTICAN-2的表达存在miRNA依赖的正相关性。表明SPOCK1可以与家族其他成员之间通过ceRNA调控影响下游分子网络。我们拟研究SPOCK1及其ceRNA共同结合的miRNA并确定其核心区，分析其影响胆囊癌转移的信号通路和分子网络，阐明SPOCK1及其ceRNA在胆囊癌细胞转移过程中的分子机制，以拓展对胆囊癌转移分子机制的认识。

The regulation of cell plasticity plays a key role in GBC metastasis. Our research group has relized that RNAs can regulate each other through their ability to compete for microRNA and these RNAs with similar functions constitute a molecule group of competing endogenous RNA (ceRNA). In our previous work, we found that SPOCK1 is a key functional gene regulating the cell plasticity of metastasis in gallbladder cancer. Furthermore, we found that SPOCK1 can promote metastasis and epithelial-mesenchymal transition of gallbladder cancer. By analysis of bio-informatics and microarray, we found that TESTICAN-2 which belongs to SPARC family is the ceRNA of SPOCK1 gene on the base of the sharing mechanism of miRNAs. It suggests that SPOCK1 and other member of SPARC family of proteins constitute a molecule group by the regulation of ceRNA. We try to find the miRNA which can be binded to both SPOCK1 and its ceRNA and find their core region, analyze their pathways and molecular network, which uncover the functional role and regulation mechanism of SPOCK1 ceRNA, and that will contribute to the understanding the mechanism of tumor metastatic plasticity in gallbladder cancer.