丝/苏氨酸蛋白激酶RIP的七个家族成员具有不同的功能结构域。RIP1，2，3在NF-κB活化，炎症因子产生，细胞凋亡，细胞坏死等多种免疫相关效应的信号传递过程中发挥关键作用；而RIP4，5，6，7的生理功能尚不明确。表达I 型干扰素是细胞抵抗病毒侵染的主要效应之一。本项目运用shRNA和CRISPER-Cas9技术在细胞水平上分别敲低及敲除RIP1-7，研究RIP家族成员对病毒诱导I型干扰素表达的作用，检验RIPs在抗病毒不同信号通路中的功能并解析其作用机制。

The seven members of the serine/threonine protein kinase RIP family have different functional domains. RIP1, 2, 3 play a key role in the signal transmission process of NF-kappa B activation, inflammatory cytokines production, apoptosis, necrosis and other immune responses. The physiological functions of RIP4, 5, 6 are unclear. Expression of type I interferon is one of the main effect of the cells resistant to virus infection. Using shRNA/CRISPER-Cas9 to knock down / knock out RIP1-7 in cells, we plan to identify the function and analyze the mechanism of RIPs in the expression of antiviral type I interferon.