磁共振成像（MRI）是体内分析的有效手段。与传统的1H-MRI相比，19F-MRI由于零背景成为近期的研究热点。本项目拟将氟硼酸盐作为氟源，设计合成一系列新型的19F-MRI纳米探针，在活体水平实现基质金属蛋白酶（MMP）的原位分析。具体实施中，我们将利用空间位阻引起的T2弛豫增强效应，构建MMP的响应探针。同时结合动力学分析，优化探针设计，提高灵敏度和可靠性，并在细胞水平和小鼠肿瘤模型上验证其对MMP的检测效果。本项目旨在探究以氟硼酸盐作为氟源构建19F-MRI纳米探针的可行性和实施方式，并将其应用于体内MMP的成像分析。为推动基于氟硼酸盐的19F-MRI体内分析奠定实验基础和理论依据。

Magnetic resonance imaging (MRI) has become a powerful technique for in vivo analysis with high spatial resolution and effective tissue penetration. Nowadays, 19F-MRI has become research hot spot because of its negligible background signal. In this project, we propose to take fluoroborate as 19F source, design and synthesize new-type MRI probes for in vivo matrix metalloprotease (MMP) analysis. During the implementation, we will utilize the enhanced T2 relaxation effect to construct MMP responding probes. We will perform detailed kinetic to optimize the nanoprobes to promote the sensitivity and reliability, meanwhile evaluate them in cellular levels and mouse tumor models. This project intends to explore the feasibility and the method of constructing 19F-MRI probes based on fluoroborate, and then apply them to in vivo MMP analysis. That may furnish the basic theories and experimental practices for in vivo 19F-MRI analysis based on fluoroborate.