预水解是公认为最具工业化基础和应用前景的木质纤维预处理技术，可为生物质精炼提供大量高附加值半纤维素衍生物。然而在高温酸性条件下，解聚溶出的木素分子易于转化为惰性缩合型结构，对后续脱木素、酶解糖化和木素精炼转化造成障碍。本课题以高频连接的β-O-4断裂机理研究为切入点，以提取磨木木素与模型物为参照，探索预水解强度对液固两相特征解聚产物与化学键形成的影响规律，通过对α位缩合型特征结构及与β-O-4断裂密切相关的希伯特酮/甲醛的识别与定量，阐明β-O-4断裂机理及β位缩合型结构木素的形成途径，揭示苄基碳正离子对促进β-O-4深度断裂与缩合型木素结构形成的竞争机制与差别化作用规律；在此基础上，采用连续动力学方法，构建苄基碳正离子清除的木素解聚-溶出-缩合动力学模型，阐明预水解过程中苄基碳正离子清除与预水解强度对缩合型结构形成的协同控制机制，为推动“预水解+”工业模式快速发展提供理论依据和技术支撑。

Pre-hydrolysis is generally recognized as a pretreatment technique with well industrial basis and application prospect, which can provide large amount of high value-added hemicellulose degrading derivatives for biomass refining. However, in the high temperature and acidic process,the lignin molecule dissolved by depolymerization is easily converted into an inert condensation structure, which will cause obstacles in the subsequent delignification, enzymatic saccharification and lignin refining and conversion. This project is beginning with the investigation of cleavage mechanism of high frequency bonding of β-O-4 linkage in the lignin as a breakthrough point, with extracted milled wood lignin and model as reference, to explore the effect of prehydrolysis intensity on the identification and quantification of characteristic depolymerization products and chemical bonds in the liquid-solid two phase, through the identification and quantification of the α-condensation type structure and the Hibbert ketone/formaldehyde closely related to the cleavage of β-O-4 linkage, to clarify the cleavage mechanism of β-O-4 linkage and evolution path of β-condensed structure lignin, the competitive mechanism and differentiated influence will be revealed between the promotive effect through the formation of benzyl carbocation for the deep cleavage of β-O-4 linkage and the formation of condensed structure lignin ; On this basis, the isothermal depolymerization and condensation reaction kinetic model of lignin for benzyl carboncation scavenging was constructed by continuous kinetics method, and the mechanism of synergistic control of benzyl carboncation scavenging and pre-hydrolysis intensity on the formation of condensation structure during pre-hydrolysis was clarified, which will provide theoretical basis and technical support for the rapid development of “pre-hydrolysis+” biomass refining industrial model.