大脑皮层发育的有序性是执行哺乳动物高级生理功能的基础，而这个过程需要精确的基因表达调控。以表观遗传分子为切入点来探讨该过程中的功能和机制将为揭示大脑皮层发育的调控网络提供新的突破口。在本实验室的前期工作中发现TrxG家族核心成员之一的ASH2L在神经前体细胞中高表达，而且利用小鼠端脑特异表达的D6-Cre在大脑皮层发育早期特异性敲除Ash2L后发现小鼠大脑皮层发育出现异常，但具体的作用机制尚不知晓。因此，本研究拟结合前期工作深入探索ASH2L在大脑皮层发育过程中的具体作用，并深入挖掘在此过程中具体分子调控机制，以此来寻找出小鼠大脑皮层发育过程中不同类型神经元命运决定的核心调控网络，为揭示小鼠大脑皮层发育过程中神经元命运决定的调控网络。

The accurate generation of an appropriate number and subtypes of different neuron and glial is fundamental to normal brain functions and requires tightly orchestrated spatial and temporal developmental programs. Characterizing the genetic of these programs that specify development of the cerebral cortex is a central challenge in neuroscience, that has not been fully elucidated yet. Using epigenetic molecules as a starting point to explore its function and mechanism in this process will provide a new breakthrough in revealing the regulatory network of cerebral cortex development. In our previous work in our laboratory, we found that Ash2L, one of the core members of the TrxG family, is highly expressed in the neocortex of developing mouse brains and is necessary for the development of the cerebral cortex. However, the mechanism responsible for these defects need to further explored. Therefore, this study intends to clarify the spatio-temporal expression pattern of ASH2L during the neocortical development in mice, and to explore the functions of ASH2L in the developing neocortex though in vivo knockout study, which mediated by D6-Cre, a cre line begins specifically expressed in mouse telencephalon at E10.5. Then, combined with RNA-Seq, ChIP-Seq and other bioinformatics methods to systematically search for specific regulatory genes of ASH2L in this process, and through cultured neural stem cell and in utero electroporation and other approaches to identify the functional and direct downstream genes regulated by ASH2L. These results will provide new clues to reveal the core regulatory network in determine the neuronal fate of mouse cerebral cortical development.