两个新的拟南芥SWI/SNF染色体重塑复合体亚基的鉴定及其调控植物发育的机制研究

SWI/SNF染色体重塑复合体是由多个蛋白亚基组成的，通过调节染色体的结构来调控基因转录的重要蛋白复合体。SWI/SNF复合体在调节植物生长发育、生物与非生物应答等方面均起重要的作用。然而植物SWI/SNF复合体含有多少个蛋白亚基、每个亚基在复合体内具体所扮演的角色、以及它们调控植物发育的机制依然很不清楚。申请人前期用植物SWI/SNF染色体重塑酶BRAHMA作为诱饵，获得2个全新的同源的SWI/SNF互作蛋白Novel SWI/SNF Subunit 8 (NSS8)和NSS9。本项目拟系统研究NSS8/9是否是植物SWI/SNF复合体的新亚基、解析它们在复合体中所扮演的角色、阐明它们在调节植物营养生长转型过程中的遗传与分子机制。本项目的完成将增加人们对植物SWI/SNF染色体重塑复合体亚基组成的认识，同时本项目对新亚基的功能机理解析将加深人们对染色体重塑蛋白调节植物发育的机理的理解。

SWI/SNF chromatin remodelling complexes are multi-protein machineries that regulate gene transcription by regulating chromatin structure. SWI/SNF complexes are essential for plant development and response to biotic and abiotic stresses. However, how many subunits plant SWI/SNF complexes contain, the exact role of each subunit in the complex, and the mechanism of them in regulating plant development are still poorly understood. We previously performed immunoprecipitation-mass spectrometry using SWI/SNF chromatin remodeler BRAHMA as bait and identified two novel SWI/SNF Subunit 8 (NSS8) and NSS9. In this proposal, we propose to investigate whether NSS8/9 are novel subunits of plant SWI/SNF complexes, reveal the roles of NSS8/9 in the complexes, and uncover the molecular mechanism by which NSS8/9 regulate vegetative phase transition. This work will renew our understanding on the composition of plant SWI/SNF complexes and enhance our knowledge on the mechanism of plant chromatin remodelers in regulating plant development.