拟南芥染色体重塑酶BRAHMA相互作用蛋白 BRIP1/2调节植物发育的分子机理研究

染色体重塑复合体通过改变染色质结构来调节基因转录,确保细胞内各种生物学过程的正确运行。和其它真核生物类似，植物染色体重塑蛋白通过调节相关靶基因的表达来控制植物的发育，然而其识别靶基因的分子机理依然不清楚。申请人前期的研究发现植物SWI/SNF染色体重塑酶BRAHMA(BRM)对靶基因的识别部分依赖于组蛋白去甲基化酶REF6,暗示还有其它未知的因子参与对BRM的招募。通过对我们前期BRM免疫沉淀蛋白质谱数据的分析，我们获得了2个全新的同源的BRM相互作用蛋白BRM Interacting Protein 1 (BRIP1)和BRIP2。本项目拟通过综合运用分子生物学、遗传学、表观遗传组学等手段，系统研究BRIP1/2在植物SWI/SNF染色体重塑复合体中所起的角色，阐明它们是否以及如何影响SWI/SNF复合体的装配及对靶基因的识别，解析它们在调节开花时间上的作用机理。

Chromatin remodelling complexes regulate gene transcription activity by changing the structure of chromatin, which in turn assures the proper biological function in eukaryote cells. Similar to other eukaryotes, plant chromatin remodelling complexes mediate plant development through regulating the transcription of target genes, however, the molecular mechanisms by which they recognize target genes are still unclear. Our previous work demonstrated that the binding of plant SWI/SNF chromatin remodelling complex to DNA partially depends on histone demethylase REF6, suggesting that other unknown factors are also involved in recruiting BRM. Through analyzing our previously published BRM immunoprecipitation-mass spectrometry data, we have identified two novel BRM Interacting Protein 1 (BRIP1) and BRIP2. In this proposal, we propose to combine molecular biology, genetics, and epigenomics to investigate the roles of BRIP1/2 in plant SWI/SNF complexes, uncover whether and how they affect the assemble of SWI/SNF complex and the recognition of the complex to its target genes, reveal the functional roles of the complex in regulating flowering time.