骨质疏松症（OP）是常见病，多从抗骨质疏松治疗，成骨机制在治疗中起主导作用，而成骨细胞接收应力刺激并转导为化学信号影响成骨机制。这对骨组织再生和细胞损伤修复至关重要。MC3T3-E1细胞作为力学刺激敏感细胞，是研究成骨机制经典细胞。研究表明，适度持续静压力可有效防治OP，过度刺激又会导致骨组织疲劳和显微损伤。如何找到平衡点是关键。固本增骨颗粒治疗OP疗效肯定，但机理不明。研究拟建立“固本增骨颗粒对持续静压力下MC3T3-E1细胞的成骨与应力损伤修复机制可能分别与干预BMP/TGF-β信号通路和细胞骨架重构密切相关”假说，建立模型，用LSCM、ELISA、WB、PCR等技术检测MC3T3-E1中成骨指标及持续静压力下BMP/TGF-β信号通路和细胞骨架相关因子的基因蛋白表达，拟证实固本增骨颗粒对持续静压力下MC3T3-E1细胞成骨协同效应和应力损伤修复机制，为开发防治OP中成药提供理论基础。

Osteoporosis is one of the common clinical diseases. At present, it is mainly studied from the aspect of anti-osteoporosis. Osteogenic mechanism plays a leading role in the treatment of osteoblasts, after receiving stress stimulation,osteoblasts convert it into chemical signals and affect the mechanism of osteogenesis. it is a great significance to study the osteogenesis mechanism of osteoblast on the researchof bone tissue regeneration and osteoblast injury repair. As a stress stimulating sensitive cell, MC3T3-E1 cell is often used as classical cell models for the study of osteoblast differentiation and bone metabolism.Studies have shown that moderate sustained static pressure can effectively prevent and control OP, and excessive stimulation will lead to bone tissue fatigue and microscopic damage. How to find the balance point is the key.The effect of Gu Ben Zeng Gu granule in the treatment of OP is positive, but the mechanism is unclear.Study intends to establish hypothesis that " Gu Ben Zeng Gu granule for osteogenesis and stress damage repair mechanisms of MC3T3 E1 - cell under continuous static pressure may be closely related to the intervention of BMP/TGF–β under constant static pressure signaling pathway and cytoskeleton reconstruction" . To establish the model, the osteogenic index and the gene protein expression of BMP/TGF-β signaling pathway and cytoskeleton correlation factor in MC3T3-E1 was detected by using LSCM, ELISA, WB, PCR and other techniques. It is proposed to confirm the osteogenesis synergies.and stress damage repair mechanism of MC3T3 E1 - cell under continuous static pressure and provide a theoretical basis for the development and prevention of Proprietary Chinese medicine for the Osteoporosis.