普通菜豆EXO70基因枯萎病抗性分子机制解析及其优异单倍型鉴定

普通菜豆镰孢菌枯萎病是由尖镰孢菌菜豆专化型引起的一种真菌性土传病害，是造成普通菜豆大幅度减产的重要原因。EXO70是组成植物胞泌复合体的关键亚基之一，参与植物抗病反应。EXO70亚基通过诱导植物细胞内水杨酸含量的升高，激活植物抗病相关基因的表达，从而提高寄主对病原菌的抗性。项目组前期根据转录组测序结果挖掘到普通菜豆胞泌复合体亚基编码基因PvEXO70，并克隆到该基因。本项目拟在此基础上，采用基因过表达、基因沉默和基因组编辑技术验证PvEXO70基因功能，利用蛋白质互作技术解析其与胞泌系统其它因子的互作关系，通过多组学联合分析技术解析PvEXO70基因参与的抗病分子调控网络，应用单倍型分析技术明确优异单倍型衍化规律和地理分布特点，鉴定优异单倍型，开发可应用于分子育种的功能标记。本项目的实施将为普通菜豆镰孢菌枯萎病抗病性的改良、抗病种质创新和抗病分子机制的研究奠定理论基础。

Fusarium wilt caused by Fusarium oxysporum f. sp. phaseoli (Fop) is one of the typical fungal soil-borne diseases of common bean (Phaseolus vulgaris L.). It causes the severe yield reduction of common beans in China. EXO70 is a key subunit of plant exocyst complex, and the EXO70 subunit is involved in the plant resistance. The EXO70 subunit activates the expression of the resistance-related genes by increasing the level of salicylic acid (SA) in plants, and enhances host resistance to pathogens. We have discovered a gene named by PvEXO70 encoding a subunit of exocyst complex in common beans according to the previous results of transcriptome sequencing, and cloned the PvEXO70 gene. This study includes that the bio-function of PvEXO70 gene is identified through overexpression, gene silencing and genome editing technology. The interaction relationship between PvEXO70 and other key factors involved in the exocytosis is analyzed by the protein-protein interaction experimental technology and we further use multi-omics joint analysis technology to reveal the PvEXO70-mediated molecular regulatory network. The haplotypes analysis technology is used to clarify the derivation and geographical distribution of the PvEXO70 excellent haplotypes and to identified the excellent haplotypes to develop the function markers applied in molecular breeding. The project implementation can provide a theoretical basis for the study of the resistance molecular improvement, the resistant germplasm innovation and the resistance molecular mechanism against Fusarium wilt in common beans.