Genome-wide identification of the TGA genes in common bean (Phaseolus vulgaris) and revealing their functions in response to Fusarium oxysporum f. sp. phaseoli infection.
Genome-wide identification of the TGA genes in common bean (Phaseolus vulgaris) and revealing their functions in response to Fusarium oxysporum f. sp. phaseoli infection.
复制标题
全基因组对普通豆（叶状球叶）中TGA基因的鉴定，并揭示其对氧气孢子菌的响应f的功能。 sp。菜果感染。
DOI：
10.3389/fgene.2023.1137634
复制
发表时间：
2023
影响因子：
3.7
通讯作者：
Xue, Renfeng
中科院分区：
文献类型：
--
作者：
Liu, Yu;Huang, Yuning;Li, Zhao;Feng, Ming;Ge, Weide;Zhong, Chao;Xue, Renfeng
菜豆枯萎病是由尖孢镰刀菌引起的世界性病害。菜豆（Phaseoli（Fop）.）有必要有功能的基因，以应对Fop感染，因为他们可能会被用来管理疾病。TGA结合转录因子（TGA）作为一种重要的调节因子，参与植物对病原菌的防御机制。然而，TGA调节剂在菜豆中响应Fop感染的作用尚未得到记录。因此，我们在菜豆中进行了全基因组鉴定和特征化的8个TGA基因。在这项研究中，8个PvTGA基因分布在6条染色体上，分为4个亚组。PvTGA基因具有相同的保守的bZIP和DOG1结构域，但在不同的PvTGA中存在特定的序列结构。系统发育分析和同线性分析表明，PvTGA基因与豆科植物的TGA具有较近的亲缘关系，PvTGA 03和PvTGA 05可能在进化中起重要作用。转录组数据解释了PvTGA基因在不同组织中的表达水平存在差异。接种Fop后，抗病基因型和易感基因型之间PvTGA 03和PvTGA 07的表达水平存在显著差异。在SA处理下，PvTGA 03、PvTGA 04、PvTGA 06、PvTGA 07和PvTGA 08的表达量差异显著。这些结果表明，PvTGA 03和PvTGA 07在SA介导的枯萎病抗性中起关键作用。总之，这些发现推进了菜豆中PvTGA基因家族的知识，并将有助于未来旨在减少枯萎病的研究。
Fusarium wilt, which affects common bean all across the world, is caused by Fusarium oxysporum f. sp. Phaseoli (Fop). It is necessary to have functional genes in response to Fop infection because they might be used to manage disease. As a crucial regulator, TGA-binding transcription factor (TGA) is engaged in the defense mechanism of plants against pathogens. The role of TGA regulators in common bean in response to Fop infection, however, has not been documented. Hence, we performed genome-wide identified and characterized eight TGA genes in common bean. In this study, eight PvTGA genes were distributed on six chromosomes and classified into four subgroups. The PvTGA genes have the same conserved bZIP and DOG1 domains, but there are specific sequence structures in different PvTGAs. Phylogenetic and synteny analysis explained that PvTGA gene has a close genetic relationship with legume TGAs and that PvTGA03 and PvTGA05 may play an important role in evolution. Transcriptome data explained that expression levels of PvTGA genes showed diversity in different tissues. After Fop inoculation, the expression levels of PvTGA03 and PvTGA07 were significantly different between resistant and susceptible genotypes. Under SA treatment, the expression levels of PvTGA03, PvTGA04, PvTGA06, PvTGA07 and PvTGA08 were significantly different. These results imply that PvTGA03 and PvTGA07 play key roles in SA-mediated resistance to Fusarium wilt. Together, these findings advance knowledge of the PvTGA gene family in common bean and will help future studies aimed at reducing Fusarium wilt.
登录
查看更多内容
影响因子：
7.2
作者：
Cheng, Chia-Yi;Krishnakumar, Vivek;Town, Christopher D.
通讯作者：
Town, Christopher D.
影响因子：
11.6
作者：
Fulton, TM;Van der Hoeven, R;Tanksley, SD
通讯作者：
Tanksley, SD
影响因子：
64.8
作者：
Jaillon, Olivier;Aury, Jean-Marc;Wincker, Patrick
通讯作者：
Wincker, Patrick
影响因子：
64.8
作者：
KATAGIRI, F;LAM, E;CHUA, NH
通讯作者：
CHUA, NH
影响因子：
6.5
作者：
Idrovo Espin, Fabio Marcelo;Peraza-Echeverria, Santy;Santamaria, Jorge M.
通讯作者：
Santamaria, Jorge M.