精子激活是动物受精完成的先决条件。精子是终端分化细胞，精子激活依赖于质膜上离子通道，调控细胞内pH(pHi)、膜电势及胞内Ca2+浓度变化，进而使精子从静止状态进入快速运动状态。尤其胞内Ca2+是驱动鞭毛振动，调节精子运动行为以完成受精作用的最关键要素。以往Ca2+通道在精子激活中作用的研究，多集中于体内受精哺乳动物及体外受精硬骨鱼类，而有关体内受精硬骨鱼类精子如何感知环境Ca2+变化，并将信号传导至细胞内部，进而调控精子激活运动的过程等相关研究匮乏。因此，本项目拟以许氏平鮋为研究对象，结合生物信息学分析，CASA精子分析系统和膜片钳电生理技术，通过鉴定精子特异Ca2+通道，分析Ca2+通道电生理特性及在体内精子激活中的作用，解析Ca2+通道在许氏平鮋体内精子激活中的作用机制，为体内受精硬骨鱼类精子获能及受精提供基础资料，完善精子适应性进化的基础理论。

Sperm activation is a prerequisite for the completion of fertilization in animals. Spermatozoa are terminally differentiated cells. Sperm activation is dependent on ion channels in the plasma membrane of spermatozoa and regulates changes in intracellular pH (pHi), membrane potential, and intracellular Ca2+ concentration, which in turn causes the spermatozoa to enter a fast-moving state from a resting state. In particular, intracellular Ca2+ is the most crucial element that drives flagellar vibration and regulates sperm motility to complete fertilization. Previous studies have focused on internal fertilization mammals and external fertilization teleost, but little information related to internal fertilization teleost about how sperm senses environmental Ca2+, and transmit signals to the interior of cells, thereby regulating the process of sperm activation. This project intends to use Sebastes schlegelii as the research object. Therefore, this project intends to identify sperm-specific Ca2+ channels，analyze the electrophysiological characteristics and the role during spermatozoa activation in vivo by combining bioinformatics analysis, CASA sperm analysis system and patch clamp electrophysiology technology. Clarify the mechanism of Ca2+ channel in the spermatozoa activation in vivo of Sebastes schlegelii. The present study provides basic data on sperm capacitation and fertilization for internal fertilization teleost and improves the basic theory of adaptive evolution of sperm.