HXK介导外源糖调控采后青花菜PCD的机制研究

细胞程序性死亡（PCD）在植物正常生长发育和胁迫反应中发挥着重要作用，但在采后生物学领域，果蔬PCD的调控机制研究鲜有报道。本课题在前期研究基础上，利用分子生物学和细胞生物学等技术，从细胞超微结构、蛋白质功能和DNA变化等三个不同方面证实青花菜衰老过程中可能存在的PCD事件，探究己糖类似物和HXK抑制剂对青花菜细胞核DNA断裂、线粒体中细胞色素C释放、活性氧和能量代谢的影响，揭示己糖激酶介导糖在青花菜PCD启动、发生和发展过程中的作用和可能调控模式。本课题扩大了PCD在采后领域的研究范围，为建立植物PCD与其它采后衰老品质调控路径提供了理论基础，也丰富了PCD理论，为果蔬采后保鲜技术的发展提供了新思路。

Programmed cell death (PCD) plays an important role during development and the stress response in plant, however, the regulatory mechanisms of PCD in postharvest fruits and vegetables remain to be further investigated. Based on our previous results, new technologies in molecular biology and cytobiology were applied to confirm that PCD event involved in the senescence of broccoli, through cyto-ultrastructure, protein function, DNA fragmentation analysis. The effects of hexose analogues and inhibitor of hexokinase (HXK) treatments on DNA fragmentation, release of cytochrome C from mitochondria, reactive oxygen species and energy metabolism in broccoli were explored to elaborate the impact and possible modulation pattern of HXK on the launch, occurrence and development of PCD. This attempt would make a meaningful contribution to development of PCD theory and provide a new model to investigate the mechanism that regulate PCD and other senescence quality, as well a new research concentration in postharvest preservation technologies.