线粒体的功能状态影响卵母细胞成熟及发育能力，线粒体ATP敏感性钾通道（mitoKATP）能有效调控线粒体功能。为此，本项目针对“mitoKATP如何影响卵母细胞成熟”这一科学问题，开展卵母细胞体外成熟质量及线粒体功能相关研究。在猪卵母细胞体外成熟过程中，通过mitoKATP的特异性开放剂和抑制剂调控其开闭状态，从减数分裂进程、细胞周期因子表达、氧化还原状态、细胞凋亡以及发育能力分析卵母细胞的核、质成熟；从线粒体的亚显微结构、空间分布、膜电位强度、mtDNA拷贝数、ATP合成、活性氧、游离钙含量、通透性转换孔 (mPTP) 状态等方面评价线粒体功能的变化情况。上述研究结果将为阐明mitoKATP在卵母细胞成熟中的作用机制提供重要理论依据，同时也丰富了mitoKATP的认知。

The functional statuses of mitochondria play a pivotal role in the process of maturation and development of oocytes. Mitochondrial ATP sensitive potassium channel (mitoKATP) effectively modulates mitochondria function. Thus, this study investigates the question how mitoKATP takes effect on oocytes maturation. We are planning to switch mitoKATP -on or -off by specific small molecular activator or inhibitor, and then evaluate the markers of oocytes maturation, such as meiosis resumption, cell cycle regulators, oxidative stress, apoptosis and developmental competence of oocytes. Mitochondria function were evaluated by some characters, such as ultrastructure, distribution, membrane potential, mtDNA copy number, ATP content, reactive oxygen species, Ca2+ level and mitochondrial permeability transition pore (mPTP) status. Our study will provide reliable base in theory for clarifying the mechanism of mitoKATP in oocyte maturation, and also enrich the intension of mitoKATP knowledge.