卵黄蛋白原（Vg）是非哺乳类卵生动物卵黄蛋白Vn的前体，可被裂解为卵黄高磷蛋白（Pv）和卵黄磷脂蛋白（Lv）储存在卵子中，为胚胎发育提供主要营养和能量来源。深入探讨Vg及其降解产物的形成、转运以及相关功能，对阐明卵生动物胚胎发育机制具有极其重要的意义。中华绒螯蟹是我国重要水产养殖物种之一，其生殖生物学方面的研究一直备受关注，但关于Vg合成和转运的分子调控机制尚不清楚。为此，本项目拟根据已获得的中华绒螯蟹性腺转录组及全基因组数据，筛选并验证与Vg合成和转运相关的基因，利用染色质免疫共沉淀（ChIP）和凝胶迁移（EMSA）等技术，确定FOXL2转录因子调控的靶标CYP及作用位点，分析FOXL2和FTZ-F1对靶标CYP的调控方式，CYP对性类固醇激素合成的调控，激素-受体复合物对Vg表达的调控，以及VgR介导的内吞作用机制，初步阐明中华绒螯蟹Vg合成和转运的分子机理。

Vitellogenin(Vg), as a female-specific reproductive related protein, is the precursor of vitellin(Vn) in oviparous non-mammalian. Vg can be cleaved into phosvitin (Pv) and lipovitellin (Lv), stored in egg, providing the nutrients and energy for developing embryos. Study on synthesis, transport and function of Vg and its degradation products is extremely important to elucidate the embryonic development mechanism of oviparous animal. The Chinese mitten crab is one of the important aquaculture species, the research on reproductive biology has been great concerned. However, the molecular mechanism of Vg synthesis and transport is unclear. Therefore, this proposed project is to scree the genes related to synthesis and transport of Vg, and verify the sequence, based on the gonadal transcriptome and genome-wide data. Using ChIP, EMSA and other techniques, to verify the target CYP and the specific binding sites regulated by FOXL2 transcription factor, and to analyze the regulation of FOXL2 and FTZ-F1 onCYP, CYP on sex steroid hormone synthesis, hormone-receptor complex on Vg, and VgR-mediated endocytosis mechanism, preliminary elucidating the molecular mechanism of Vg synthesis and transport in the Chinese mitten crab.