单颗粒低温透射电镜技术发展至今，最大的瓶颈在于样品制备。而样品制备不可或缺的耗材是透射电镜载网。透射电镜载网（特别是覆在其上的支持膜）的亲水性能，机械性能，导电性能等直接影响着制备样品的质量及数据收集效率。本项目在已自主研制并应用的GIG微阵列碳支持膜的基础上，继续研发更符合实际需要的特定厚度、孔径及孔间距的微阵列支持膜，并以提升支持膜的导电性为目标以开发出第二代的该系列微阵列支持膜。最终将第二代的微阵列支持膜应用到低温透射电镜技术中，降低电子束引发的图像漂移状况，提升单颗粒低温透射电镜成像质量和数据收集效率。

Single particle cryo electron microscopy develops very fast these years. The bottle neck of this technique is at sample preparation. Among all those important factors of sample preparation, e.g. protein purity, concentration, rigidity et al., the properties of TEM grid, to be specific, the properties of supporting film on the TEM grid, is essential to preparing a good cryo sample. Hydrophilicity, strong mechanical strength, high conductivity of the supporting film affect the quality of prepared cryo sample and the efficiency of data collection. Based on the self-developed amorphous carbon film with fixed-pattern micro array (brand name "GIG"), this study aims to develop more suitable supporting films for cryoTEM usage. It includes designing suitable film thickness, hole size, spacing between hole which leads to high efficiency data collection, and increasing the conductivity of the supporting film at liquid nitrogen temperature which reduces the beam induce movement and enhances the quality of cryoEM images.