种皮颜色由种皮色素类型和含量决定，是种子的重要表型性状之一，与种子休眠、种子寿命、含油量、蛋白含量、纤维素含量等重要农艺性状有关。经典遗传研究表明种皮颜色调控方式主要分为母体效应和花粉效应（花粉直感，种胚直感）。遗传育种中花粉直感效应基因表型比母体效应基因表型早一个种植世代，具有特殊的应用价值。本研究在筛选获得花粉效应基因调控油菜黄籽性状的单基因突变体的基础上，结合经典遗传分析、转录组测序和60K油菜SNP芯片作图，将该基因定位于C4染色体3.2M的区段，转录组数据表明该区域只有一个基因表达有差异，另外尚有81个无表达差异的基因被转录组测序检测到。本研究结合精细定位和候选基因多态性分析确定候选基因，通过转基因的方法进行基因功能验证。为与应用研究相结合，本研究将雄性不育基因和候选基因构建在同一转化载体上，尝试构建黄籽-不育连锁体系，开拓杂种优势利用的新途径。

Seed coat colour is determined by the type and content of pigment deposited in the seed coat cells. It is related with many important agronomic traits of seeds, such as seed dormancy, longevity, oil content, protein content and fibre content. Conventional genetic research shows that genetic control of seed coat colour is composed of maternal effect and pollen effect (xenia effect). Because phenotype of genes with pollen effects are exhibited one growing season ealier than the phenotype of genes with maternal effects, this kind of genes have specific useness in the breeding. For example, we can link the yellow seed coat gene with pollen effect together with male sertrile genes to construct yellow seed coat indicated male sterile lines for hybrid producing. .In this research, we isolated a mutation of yellow seeded Brassica napus line controlled by single mendel locus with pollen effect. Using the Brassica 60K Infinium BeadChip SNP genotyping array, we mapped the yellow seed coat gene on C4 genetic linkage map. The linked makers flank the gene within about 3.2M physical region.Using RNAseq technology, we accquired 50K unigenes transcripted in the seeds and 53 genes differently transcripted between brown wild type and yellow mutation. Only one of the 53 genes located on the 3.2M gene-mapped region. Another 81 transcripted genes accquired by RNAseq were loacted in the region with SNP polymorphism. Based on these work, this project is intergating fine-mapping and bioinfomatics to isolate the candidate genes, then verifys the gene function by genetic transformaiton. In order to intergate the breeding research, we are linking some male sterile gene to the candidate gene to construct yellow seed coat-male strile linked breeding lines.