云南省宣威地区农村女性肺癌发病率居世界首位，独特的生活环境使宣威成为我国非吸烟女性肺癌高发现场。由于缺乏突破性的研究证据来帮助政府制定相关防治策略，宣威地区农村女性肺癌仍然呈现升高趋势和地域扩大化的弥漫态势。我们研究发现，宣威地区农村女性长期吸入含纳米铁颗粒的煤烟尘导致体内铁过载，体内铁过载可通过调节细胞铁死亡信号通路与癌症发生密切相关。本项目通过生物样本实验，在宣威农村女性肺癌患者中寻找与“铁过载”和 “细胞铁死亡”发生的生物学证据，为宣威地区肺癌高发机制研究提供思路；通过体外细胞和动物铁过载实验，应用siRNA技术和pEGFP-C3过表达载体技术，从细胞微观形态、分子、基因和蛋白水平多角度研究“体内铁过载”与“细胞铁死亡”信号通路在宣威地区农村女性肺癌发生发展中的作用，为“体内铁过载--细胞铁死亡--肺癌发生”的机制研究提供参考，为宣威地区农村女性肺癌预防及治疗靶点选择提供科学依据。

Abstract: Xuanwei region in Yunnan Province have the highest incidence of lung cancer in rural women all around the world. The unique living environment makes Xuanwei a high incidence of non-smoking women's lung cancer in China. The incidence and high risk region of lung cancer in rural women in Xuanwei appear a trend of increasing, due to lack of breakthrough research evidence that helps the government formulate prevention and control strategies. Our study found that long term inhalation of coal smoke which contains iron nanoparticles in Xuanwei result in iron overload, and iron overload is closely related to the occurrence of cancer through regulating the signal pathway of ferroptosis. This project finds biological evidence of "iron overload" and "ferroptosis" in Xuanwei rural women with lung cancer through biological sample experiment, and provide train of thought for studying the mechanism of lung cancer in Xuanwei. Use iron overload experiment in vivo and in vitro, and application of siRNA and pEGFP-C3 over expression technology, to research the role of "iron overload" and " ferroptosis" pathway in lung cancer in Xuanwei rural women, from cell morphology, molecular, gene and protein level. To provide a evidence for studying mechanism of "iron overload -ferroptosis -- lung cancer", and provide a scientific evidence for prevention and selecting target for treatment of lung cancer in rural women in Xuanwei.