促进有效肝再生对提高肝衰竭患者存活率至关重要。基于肝衰竭“毒邪—毒浊”新学说形成的解毒化瘀颗粒可增强胆汁酸代谢促进肝再生。业已探明胆汁酸、FXR、肠道微生态及干细胞存在次第的分子偶联。基于此，课题组提出解毒化瘀颗粒通过胆汁酸-FXR-肠道微生态途径促进肝干细胞转化为肝细胞、增强肝再生、拮抗肝衰竭的疗效机制假说。研究中拟复制肝衰竭大鼠模型，解毒化瘀颗粒、胆汁酸合成抑制剂及抗生素复合物干预，采用16S rRNA测序、GC-MS、Western Blot及qPCR等研究技术，揭示肝衰竭进展中胆汁酸-FXR途径对肠道微生态的多层次调控效应，并建立其与肝干细胞转化的表型关联；从分子—细胞—动物层面阐释解毒化瘀颗粒通过胆汁酸-FXR-肠道微生态途径促进肝干细胞转化、调控肝再生的分子机制。洞悉解毒化瘀颗粒正向调控肝再生、拮抗肝衰竭以及中医“祛瘀生新”的科学内涵，为肝衰竭中医药治疗拓展思路及方法提供借鉴。

Promoting effective liver regeneration is the key point for improving survival rate of patients with liver failure. The formation of Jiedu Huayu granules is basing on the new theory of ‘Duxie -Duzhuo’ in liver failure, which can up-regulate the bile acid metabolism pathway and promote liver regeneration. Bile acids, FXR, intestinal microecology and stem cell exist molecular coupling. Therefore, our task group propose the therapeutic mechanism hypothesis of liver failure is that Jiedu Huayu granules could promote the transformation of liver stem cells into liver cells, enhance liver regeneration, antagonize liver failure through Bile acid-FXR-Intestinal microecological pathway. The project team will duplicate rats models of acute liver failure, interfere with the Jiedu Huayu granules, combine with the use of Bile acid inhibitor, Antibiotics(ABX) complex. By using the method of 16s rRNA gene detection technology, GC-MS, Western Blot, qPCR and another technologies, to reveal the multi-level regulatory effect of Bile acid-FXR-Intestinal microecological pathway on intestinal microecology in the progression of liver failure, to establish the relationship between hepatic stem cell and liver cells, to interpret the molecular mechanism of Jiedu Huayu granules how regulate the transformation of liver stem cells into liver cells through Bile acid-FXR-Intestinal microecological pathway from molecular-cell- animal level. Understanding the scientific connotation of Jiedu Huayu granules in regulating liver regeneration, antagonizing liver failure and “Quyu Shengxin” in TCM can provide references for expanding the thinking and methods of TCM treatment for liver failure.