搭载microRNA-132的多功能聚氨酯三维多孔支架对脑外伤后内源性脑组织再生的作用及机制研究
结题报告
批准号:
52003177
项目类别:
青年科学基金项目
资助金额:
24.0 万元
负责人:
王焱超
依托单位:
学科分类:
生物医用有机高分子材料
结题年份:
2023
批准年份:
2020
项目状态:
已结题
项目参与者:
王焱超
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中文摘要
旨在改善创伤性脑损伤(TBI)后因脑组织固有再生能力有限及微环境剧烈变化导致内源性脑组织再生效果差的难题。本课题拟设计一种以可降解水性聚氨酯为基础,联合搭载microRNA-132的季铵盐聚氨酯胶束制备多功能三维多孔支架。采用阳离子聚氨酯胶束搭载microRNA-132,并与支架结合在修复的脑组织中实现局部给药提高转染效率,调节再生微环境并促进神经细胞生长,探索包载microRNA胶束与三维多孔支架的整合方式对脑组织修复的影响。采用激光共聚焦显微镜,流式细胞术,高通量蛋白芯片和动物体内实验等,研究microRNA-132对内源性神经干细胞、星形胶质细胞、免疫细胞的调控,揭示多功能三维多孔支架调控TBI后微环境、促进内源性脑组织再生的机制,本研究将为载microRNA组织工程支架用于内源性脑组织修复提供新的思路及方法。
英文摘要
To improve poor effect of endogenous brain tissue regeneration after traumatic brain injury (TBI) due to the limited inherent regeneration ability of brain tissue and the drastic change of microenvironment. This project intends to design a multifunctional three dimensional porous scaffold based on degradable waterborne polyurethane and carrying microRNA-132 loaded quaternary ammonium salts-polyurethane micelles. These cationic polyurethane micelles will be applied to deliver microRNA-132 locally in the repaireing brain tissue to enhance transfection efficiency, regulate the regenerative microenvironment and promote the growth of nerve cells. The project will explore the integration of microRNA micelles and three dimensional porous scaffolds. The regulation of microRNA-132 on endogenous neural stem cells, astrocytes and immune cells will be investigated using laser confocal microscopy, flow cytometry, high-throughput protein chip and in vivo experiments, etc. Mechanism of which multifunctional three dimensional porous scaffold could effectively regulate the post-TBI microenvironment and promote regeneration of endogenous brain tissues will be revealed. This investigation will provide new ideas and methods for the application of microRNA modified tissue engineering scaffolds in endogenous brain tissue repair.
期刊论文列表
专著列表
科研奖励列表
会议论文列表
专利列表
Citicoline-liposome/polyurethane composite scaffolds regulate the inflammatory response of microglia to promote nerve regeneration
胞二磷胆碱-脂质体/聚氨酯复合支架调节小胶质细胞炎症反应促进神经再生
DOI:10.1007/s10853-021-06628-0
发表时间:2022
期刊:JOURNAL OF MATERIALS SCIENCE
影响因子:4.5
作者:Jingjing Lin;Xingying Song;Hang Yin;Nijia Song;Yanchao Wang;Zhen Li;Feng Luo;Hong Tan;Xueling He;Jiehua Li
通讯作者:Jiehua Li
DOI:--
发表时间:2023
期刊:Journal of Materials Chemistry B
影响因子:7
作者:Qiao Zhang;Jinlin Chen;Yuan Feng;Jingjing Lin;Jiehua Li;Yanchao Wang;Hong Tan
通讯作者:Hong Tan
DOI:10.1007/s12975-023-01212-x
发表时间:2023-11
期刊:Translational stroke research
影响因子:6.9
作者:Qiao Zhang;Jinlin Chen;Jingjing Lin;Ruichao Liang;Min He;Yanchao Wang;Hong Tan
通讯作者:Qiao Zhang;Jinlin Chen;Jingjing Lin;Ruichao Liang;Min He;Yanchao Wang;Hong Tan
DOI:DOI: 10.1039/d2tb02679a
发表时间:2023
期刊:Journal of Materials Chemistry B
影响因子:7
作者:Yanchao Wang;Jingjing Lin;Jinlin Chen;Ruichao Liang;Qiao Zhang;Jiehua Li;Ting Lan;He Xueling;Hong Tan;Xuhui Hui
通讯作者:Xuhui Hui
Biodegradable polyurethane nerve guide conduits with different moduli influence axon regeneration in transected peripheral nerve injury
不同模量的可生物降解聚氨酯神经导管影响周围神经横断损伤的轴突再生
DOI:10.1039/d1tb01236c
发表时间:2021
期刊:Journal of Materials Chemistry B
影响因子:7
作者:Yanchao Wang;Ruichao Liang;Jingjing Lin;Jinlin Chen;Qiao Zhang;Jiehua Li;Minjin Wang;Xuhui Hui;Hong Tan;Qiang Fu
通讯作者:Qiang Fu
“多级式”植入材料用于精准内源性脑组织修复的研究
  • 批准号:
    52373296
  • 项目类别:
    面上项目
  • 资助金额:
    50.00万元
  • 批准年份:
    2023
  • 负责人:
    王焱超
  • 依托单位:
国内基金
海外基金