圆叶肿柄菊素A诱导茉莉酸通路抑制TSWV侵染的分子机制

番茄斑萎病毒危害云南多种经济作物，造成严重的经济损失，筛选并应用植物源抗病毒制剂是防控该类病害的重要措施之一。茉莉酸信号通路在应对机械伤害、咀嚼式昆虫和病原菌的侵害中起着重要作用。前期研究发现圆叶肿柄菊素A能够激发茉莉酸信号通路抑制番茄斑萎病毒侵染，但相关激发、抑制机制尚不清楚。本研究拟利用转录组学解析圆叶肿柄菊素A处理与对照烟草叶片的基因表达调控差异，通过基因沉默、双分子荧光互补、酵母双杂交等方法研究与圆叶肿柄菊素A互作激发茉莉酸信号通路的转录因子、调控茉莉酸信号通路抑制番茄斑萎病毒侵染的关键基因，揭示圆叶肿柄菊素A激发茉莉酸信号通路抑制番茄斑萎病毒侵染的分子机制。本研究将对深入理解植物茉莉酸信号通路防御病原微生物的机理具有重要的理论意义，为筛选新型植物源抗病毒化合物提供重要的理论指导。

Tomato spotted wilt virus (TSWV) causes serious losses on many economic crops in Yunnan province. Screening and application of botanical pesticide is an important way to control TSWV. Jasmonic acid (JA) signaling pathway plays key roles in response to mechanical injury, chewing insects and pathogen infection. Previous studies have proved that TagitininA (TagA) could inhibit TSWV infection by stimulating jasmonic acid (JA) signaling pathway, however, the relative mechanisms remain unclear. This project aims to analyze the differential expressed gene profiles between the TagA-induced tabacco leaves and the control leaves using RNA-Seq. Furthermore, RNAi, BiFC, yeast two-hybrid etc will be used to investigate the molecular mechanisms involving transcription factors which recognize TagA and induce JA signaling pathway, the genes in JA signaling pathway and the mechanisms which regulate JA signaling pathway to TSWV defense. The present study will enhance our understanding on the mechanism of jasmonic acid mediated signaling pathway against pathogenic microorganisms, the obtained information may serve as reference for screening of anti-virus bio-pesticides.