基于EMSCs衰老的二补助育汤对高龄鼠子宫内膜容受性调控机制的研究

临床寻求IVF-ET帮助的高龄女性日益增多，但妊娠率较低，子宫内膜容受性低是主要影响因素。前期研究证实，名老中医经验方二补助育汤能通过SCF、ανβ3、HOXA-10、eNOS等靶点改善子宫内膜容受性、提高高龄女性IVF-ET妊娠率；现代研究提示，子宫内膜间充质干细胞（EMSCs）可能通过影响子宫内膜细胞更新、组织修复及血管生成，从而参与子宫内膜容受性的建立，而子宫内膜细胞衰老与胚胎着床障碍密切相关。因此，我们提出“二补助育汤通过调控EMSCs衰老，调节子宫内膜容受性，从而提高高龄女性妊娠率”的假说，并以高龄鼠EMSCs为切入点，将体外与体内研究相结合，采用转录组学、CCK8、EDU、SA-β-gal染色法、RT-PCR等方法，证实EMSCs衰老与子宫内膜容受性的相关性，同时研究二补助育汤调控高龄鼠子宫内膜容受性的作用机理，进一步揭示中药提高高龄女性IVF-ET成功率的新作用靶点和机制。

The number of elderly women seeking the help of IVF-ET is increasing, but the pregnancy rate is low and the low endometrial receptivity is the main influencing factor. Erbu Zhuyu Decoction is an experienced Chinese medicine decoction of professor Xiao Chengzong for the treatment of low endometrial receptivity. Previous studies showed that Erbu Zhuyu Decoction could improve endometrial receptivity and pregnancy rate of women's in vitro fertilization and embryo transfer(IVF-ET) through pinopodes, SCF, ανβ3, HOXA-10, eNOS and other targets. Modern research showed that the endometrial mesenchymal stem cells (EMSCs) may participate in the establishment of endometrial receptivity by affecting endometrial cells renewal, tissue repairment and angiogenesis of endometrium.The aging of endometrial cells is closely related to embryo implantation dysfunction. Therefore, the research hypothesized that Erbu Zhuyu Decoction could regulate endometrial receptivity by regulating the aging of EMSCs, so as to improve the pregnancy rate of elderly women. Taking EMSCs as the starting point, combining in vitro and in vivo research, transcriptome, CCK8, EDU, flow cytometry, SA-β-gal staining and RT-PCR were used to confirm the correlation between the aging of EMSCs and endometrial receptivity in this study. To study the mechanism of Erbu Zhuyu Decoction in regulating endometrial receptivity in aged female mice, and further reveal the new targets and mechanisms of TCM in improving the success rate of IVF-ET in elderly women.