蕨麻多糖及其亚硒酸酯精细化学结构与抗辐射氧化损伤构效关系研究

藏区特色植物蕨麻资源丰富，有待深度研发。项目组前期初步研究蕨麻多糖（PAP）及亚硒酸酯（Se-PAP）的单糖组成、分子量、抗辐射活性。本项目针对PAP和Se-PAP精细化学结构及对抗辐射氧化损伤作用相关性，拟制备不同分子量PAP和不同取代位置、硒含量的Se-PAP，系统研究其化学结构（单糖组成和连接顺序、分子量、糖链分支点、取代基含量、位置等）与溶液构象，以及化学结构对溶液构象的影响规律；通过体内外抗辐射氧化损伤活性实验，从细胞和动物两个维度筛选、验证活性较好的PAP和Se-PAP活性簇材料。综合比较高活性PAP和Se-PAP的结构共性特征，应用计算机模拟定量构效关系（QSAR）方法，探究结构与活性之间的定量关系，识别影响PAP和Se-PAP活性的结构因素。最终阐明PAP和Se-PAP的精细化学结构与抗辐射氧化损伤构效关系研究，为多糖构效关系研究和多糖基天然辐射防护剂的开发提供科学依据。

Potentilla anserina L. is widely distributed in tibetan area, China. In our previous study, the composition, molecular weight and radioprotective effect of the Potentilla anserina polysaccharide (PAP) and selenium-containing polysaccharides (Se-PAP) were researched. Most reports had studied PAP and Se-PAP lack of systematic chemical structure, structure-activity relationship and the mechanism of anti-radiation. In this project, different molecular weight of PAP and different substituted positions of Se-PAP were prepared. Structure characterization (monosaccharide composition, linkage of glycosidic bond and branch point) of PAP and Se-PAP were investigated. Anti-radiation effects of PAP and Se-PAP (vitro, vivo and QSAR) were evaluated for a better understanding of the relationship between structure and biological response effects. It provides a scientific basis for the study of polysaccharide structure-activity relationship and the development of polysaccharides materials radiation protectant.