藏区特色植物蕨麻资源丰富，有待深度研发。项目组前期初步研究蕨麻多糖（PAP）及亚硒酸酯（Se-PAP）的单糖组成、分子量、抗辐射活性。本项目针对PAP和Se-PAP精细化学结构及对抗辐射氧化损伤作用相关性，拟制备不同分子量PAP和不同取代位置、硒含量的Se-PAP，系统研究其化学结构（单糖组成和连接顺序、分子量、糖链分支点、取代基含量、位置等）与溶液构象，以及化学结构对溶液构象的影响规律；通过体内外抗辐射氧化损伤活性实验，从细胞和动物两个维度筛选、验证活性较好的PAP和Se-PAP活性簇材料。综合比较高活性PAP和Se-PAP的结构共性特征，应用计算机模拟定量构效关系（QSAR）方法，探究结构与活性之间的定量关系，识别影响PAP和Se-PAP活性的结构因素。最终阐明PAP和Se-PAP的精细化学结构与抗辐射氧化损伤构效关系研究，为多糖构效关系研究和多糖基天然辐射防护剂的开发提供科学依据。

Potentilla anserina L. is widely distributed in tibetan area, China. In our previous study, the composition, molecular weight and radioprotective effect of the Potentilla anserina polysaccharide (PAP) and selenium-containing polysaccharides (Se-PAP) were researched. Most reports had studied PAP and Se-PAP lack of systematic chemical structure, structure-activity relationship and the mechanism of anti-radiation. In this project, different molecular weight of PAP and different substituted positions of Se-PAP were prepared. Structure characterization (monosaccharide composition, linkage of glycosidic bond and branch point) of PAP and Se-PAP were investigated. Anti-radiation effects of PAP and Se-PAP (vitro, vivo and QSAR) were evaluated for a better understanding of the relationship between structure and biological response effects. It provides a scientific basis for the study of polysaccharide structure-activity relationship and the development of polysaccharides materials radiation protectant.