精子DNA损伤修复机制的缺陷是引起男性不育的重要原因之一。其中，染色质重塑对于DNA损伤的修复至关重要。在前期的工作中，我们发现精子发生相关新基因SPATA12与染色质重塑因子CHD2存在相互作用，提示SPATA12可能参与了细胞内DNA损伤的修复。基于此，本课题的研究内容主要包括四个方面：（1）明确SPATA12与CHD2具有结构和功能性的相互作用；（2）建立DNA损伤细胞模型，阐明SPATA12在DNA损伤修复中的作用；（3）探讨SPATA12通过ATM-Chk和H2AX-p53途径参与DNA损伤修复的分子机制；（4）分析SPATA12的表达与人精子DNA损伤的相关性。通过上述研究，以期为深入认识SPATA12在精子DNA损伤修复中的功能，以及探讨男性不育的分子机制提供实验证据。

The repair-deficiency of sperm DNA damage is one of the important reasons leading to male infertility. Chromatin remodeling has been proved to be critical for DNA damage repair. In our previous study, the novel spermatogenesis associated gene 12（SPATA12）was found to be interacted with chromodomain helicase DNA binding protein 2（CHD2）,which is involved in regulating the DNA damage responses at the chromatin level. Thus, our hypothesis is that SPATA12 might play some role in DNA damage repair. In this project, our research is consisted of four parts:(1) Confirm both structural and functional interaction between SPATA12 and CHD2 protein;(2) Investigate the role of SPATA12 in DNA damage based on cellular model of DNA damage;(3) Explore the molecular mechanism of SPATA12 involved in DNA damage repair via ATM-Chk and H2AX-p53 signaling pathway;(4) Analyze the relationship between expression of SPATA12 and human sperm DNA damage. In conclusion, these studies would develop our knowledge on understanding the function of SPATA12 in sperm DNA damage repair, and provide experimental evidence for uncovering the molecular basis of male infertility.