miRNA对子宫内膜蠕动波的调控及其机制研究

子宫内膜蠕动波是一种无创性评估子宫内膜容受性的重要指标，在胚胎着床中起关键作用，但调控子宫内膜蠕动波的机制不清。miRNAs参与调节子宫内膜的各种生理活动及胚胎的植入，但目前所知的参与子宫内膜蠕动波调控的miRNA甚少。为筛选调控子宫内膜波状运动的miRNA，我们拟采用miRNA-mRNA芯片联合分析，筛选并验证调控子宫内膜蠕动波的miRNA及其靶mRNA基因；分析miRNA及其靶基因表达改变对子宫内膜间质细胞及内膜下肌层平滑肌细胞的细胞内钙离子浓度和运动的影响；并在细胞共培养模型中验证靶基因对子宫内膜蠕动波的调控功能。本研究将子宫特异表达的miRNA与子宫内膜蠕动波、子宫内膜容受性相联系，对阐明子宫蠕动波的发生机制及其对胚胎着床的影响具有重要意义，为异常子宫内膜蠕动波的临床治疗奠定基础。

Uterine peristalsis plays an important role in embryo implantation and could be a non-invasive criterion to predict endometrial receptivity. However, the mechanism of uterine peristalsis regulation is unclear.miRNAs take part in various kinds of endometrial physiological activities and implantation of embryo.While few miRNAs were known about uterine peristalsis regulation. Thus, studies are designed to find out the related miRNA and target mRNA by miRNA-mRNA microarray conjoint analysis. Influence of miRNA and its target gene expression changes to intracellular calcium ion concentration and movement of endometrial stromal cells and smooth muscle cells beneath endometrium are analyzed. The regulation role of target gene is confirmed in a cell co-culture model to clarify the mechanism of uterine peristalsis . In conclusion, this study connects uterus specifically expressed miRNA with uterine peristalsis and endometrial receptivity, has important implications for clarifying the mechanism of uterine peristalsis and its influence on embryo implantation, and lays the foundation for the treatment of abnormal uterine peristalsis.