子宫内膜容受期宫腔液外泌体miRNA在胚胎着床中的作用及调控机制初探

子宫宫腔液作为胚胎着床的微环境，内含的外泌体可以通过介导细胞间通迅，协调母胎对话，参与调节胚胎着床。miRNA是外泌体的分子内容物，也是胚胎着床的重要调控分子。在子宫内膜容受期，子宫内膜细胞选择性的将关键miRNAs包装至外泌体，并将其释放到宫腔液中，调控胚胎着床。为寻找人类子宫内膜容受期宫腔液外泌体中与胚胎植入相关的关键miRNAs，并初步探索其在胚胎着床中的作用及调控机制，我们拟采用高通量技术对人类子宫内膜容受期及非容受期宫腔液的外泌体miRNAs进行测序分析，构建差异表达谱，筛选并验证与胚胎植入信号通路相关的关键miRNAs，再用基因转染技术从功能上再次筛选关键miRNAs，得到目的miRNAs并探究其在胚胎着床中的作用及调控机制，为未来开发无创子宫内膜容受性生物标志物及临床实施个体化胚胎移植奠定基础。

As a liquid microenvironment, uterine luminal fluid(ULF) contains nano-packages called exosomes, which mediate the intercellular communication and harmonize maternal-fetus conversation to facilitate the embryo implantation. miRNAs which are proved to play an important role in embryo implantation are differentially enrich in the exosomes and potentially participate the maternal-fetus negotiation. During the endometrial receptive period, the endometria secrets exosomes, which are specifically packaged with miRNAs, into the uterine cavity fluid and regulate embryo implantation. To elucidate uterine cavity fluid exosome-miRNAs who paly important role in the implantation and uncover the mysterious mechanism, we are planning to use high-throughput sequencing to reveal the differential expression of exosome-miRNAs profile by comparing the receptive and non-receptive uterine cavity fluid derived exosomes and select the miRNA which are predicted to participate in embryo implantation signaling pathways. By using gene transfection technology, we will verify the miRNA which truly regulate embryo implantation and detail its mechanism. Result of our project will provide a great progress to the development of non-invasive endometrial receptivity biomarkers and help to guide individual embryo transfer in clinical implementation.