本研究将采用体外培养RWPE-1细胞，在LAPTM4B基因沉默或者过表达的条件下，在使用或不使用镉诱导剂的条件下，通过对RWPE-1细胞形态改变的观察，AR、MMP-2及MMP-9表达情况的检测及细胞生物学行为变化的研究来评估LAPTM4B对RWPE-1细胞增殖、分化及恶性转化的影响。检测PI3K/AKT通路相关蛋白的表达及活性，以及运用相关特异性拮抗剂探讨LAPTM4B调控下PI3K/AKT通路在RWPE-1细胞增殖、分化及恶性转化过程中的重要性。建立裸鼠动物模型，在体内水平上探讨LAPTM4B对RWPE-1细胞增殖、分化及恶性转化的调控作用，以及对RWPE-1细胞致瘤性的影响。另外我们还将研究LAPTM4B与PI3K蛋白之间相互作用的具体分子机制。为LAPTM4B可以有效调控RWPE-1细胞增殖、分化及恶性转化的假设提供科学依据，为前列腺上皮恶性转化的预防及治疗提供新的途径。

In order to investigate the real effects of LAPTM4B on RWPE-1 cell proliferation, differentiation and malignant transformation, under the situations of LAPTM4B overexpression or knockdown, morphology of the cells, the expression of AR, MMP-2 and MMP-9, and the changes of biological behavior will be studied. Expression and activity of proteins in PI3K/AKT signal pathway will be measured. Specific PI3K/AKT signal pathway inhibitors will be used to investigate the importance of PI3K/AKT signal pathway mediated by LAPTM4B in regulating RWPE-1 cell proliferation, differentiation and malignant transformation. Meanwhile, we will study the functions of LAPTM4B in the cell proliferation, differentiation, malignant transformation and carcinogenicity at animal model level. We will also examine the possible molecular mechanisms and biological functions of the interaction between PI3K and LAPTM4B. Our study will provide both the evidence of the important role of LAPTM4B in regulating RWPE-1 cell proliferation, differentiation, and malignant transformation and a novel method to prevent and treat RWPE-1 malignant transformation.