本研究拟利用纳米豆粕载运β-胡萝卜素，探索纳米食品对营养素的载运规律。纳米豆粕由豆粕经纳米加工获得，因其尺度效应而具有吸附性好、集聚性强的特点，适于作为营养素载体。研究主要包括：吸附-积聚法构建基于纳米豆粕的β-胡萝卜素载运体系，选用适宜粒径分布的纳米豆粕，优化纳米豆粕与β-胡萝卜素的配比和载运（积聚）条件，使得纳米豆粕在表面吸附β-胡萝卜素后发生积聚，将营养素包裹在积聚体中完成载运体系构建；对载运体系的形貌，载运能力、释放动态等物理化学性质进行表征；通过分析营养素的光谱学性质研究其与载体的结合方式；观察载运后β-胡萝卜素的抗氧化能力变化和小肠上皮穿透能力，考察载运后营养素的稳定性和生物利用率的变化。本项目研究探索纳米食品颗粒与营养素相互作用，揭示纳米食品营养载运体系的载运规律，可为营养素的载运提供新型载体，提高营养素的稳定性，实现营养素的高效利用，也为纳米食品的开发利用提示新的方向。

Here, nano defatted soybean was used as nutrition carrier for β-carotene delivery system. After nanofabrication, defatted soybean flour fell in nanosize, namely nano defatted soybean. The scale effect conferred the nano defatted soybean high adsorption capacity and strong aggregation tendency, which made it suitable for being the nanocarrier. .In presented proposal, three parts of work would be done. At first, an adsorption-aggregation way would be applied to construct the nano defatted soybean based β-carotene delivery system. Three aspects, the size of nano defatted soybean, the ratio of nano defatted soybean/β-carotene and constructing condition should be considered. After nano defatted soybean were adsorbed with β-carotene on the surface, they aggregated into nanoclusters, packaging these nutrition in among nanoparticles. The effect of particle size,carrier/nutrition ration would be investigated, and constructing conditions (in cluding the size of nano defatted soybean flour, ion concentration of the solution, pH value, temperature, stirring and so on) would be optimized..Secondly, the nutrition delivery system could be characterized on physical, chemical property. The carrying capacity, the amount of β-carotene that certain amount of nano defatted soybean can carry, would be quantified. And nutrition releasing dynamic would be characterized to find how much β-carotene would release from the delivery system depending on duration. The way nutrition binded in the cluster could be speculated from the detecting on its conformation, so spectroscopic methods would be applied to study the property of β-carotene..The stability and bioavailability of β-carotene are the third and most important subjects to be studied. The stability of β-carotene would be defined by its oxidation resistance. Study the DHHP cleaning ability of β-carotene in delivery system and its relationship with time duration comparing to normal β-carotene. The bioavailability of β-carotene is determined by its ability of penetrating through intestinal epithelial. The ability of penetrating through intestinal epithelial experiment would be performed in a culture Caco-2 cell model, measuring the concentration of β-carotene in one side of the cell layer after the β-carotene delivery system were put in the other side..Work in this project would explore the interaction between the nano food particle and nutrition, discover the regulation of nano food in nutrition delivery system as nanocarrier. This kind of delivery system can enhance the stability of nutrition and finally upgrade the bioavailability. This work would also offer a new instruction on development and application of nano food.