内质网(ER)-内吞体/溶酶体通过形成膜接触点互作，调控物质转运、降解、细胞器形态发生等，与多种疾病密切相关。Rab7-ORP1L-VAP形成ER-内吞体/溶酶体膜接触点. RILP（Rab7-interacting lysosomal protein)能够与Rab7和ORP1L相互作用，但其在ER-内吞体/溶酶体互作中的作用不清楚，而且RILP的表达受到不同生理、病理条件的调控，因此RILP可能动态参与调节ER-内吞体/溶酶体膜接触点形成，为此，我们将深入研究RILP对ER-内吞体/溶酶体互作的调控及其机制，揭示该调控作用的生理、病理意义。

ER interacts with lysosome through ER-endolysosome membrane contact sites, mediating membrane trafficking and morphogenesis of organelles, etc. which is closely related to multiple diseases. ER-endolysosome membrane contact sites were formed by Rab7-ORP1L-VAP interaction complex. RILP interacts with both Rab7 and ORP1L, however, it is not clear how RILP is enaged in the ER-endolysosome interaction. The expression of RILP is potentially regulated by patho-physiological conditions, therefore, RILP may dynamically regulat the formation of ER-endolysosome membrane contact site. In this project, we will try to investigate how RILP regulates ER-endolysosome interaction and the underlying mechanisms to uncover the patho-physiological significance of this regulation.