环境雌激素具有干扰体内正常内分泌系统稳定的功能。双酚A（BPA）是一类最常见的环境雌激素污染物，在水体中大量存在，威胁鱼类等水生动物的生存。弓背青鳉（Oryzias curvinotus）是中国本土小型鱼类，具有广盐性特点，是理想的监测不同盐度水域环境雌激素毒性的物种。本课题组已收集了我国南海沿岸不同水域的弓背青鳉群体，并在实验室驯养维持了不同盐度的弓背青鳉群体。本研究以三种盐度（0‰、15‰、30‰）弓背青鳉为对象，进行BPA连续世代暴露实验，探讨盐度介导的BPA对弓背青鳉毒性效应机制。包括以下内容：1）确定不同盐度下BPA的半致死浓度及安全浓度；2）分析连续世代BPA暴露对弓背青鳉生长发育、性腺分化及繁殖的影响；3）绘制弓背青鳉连续世代BPA暴露的基因和蛋白表达图谱，筛选不同盐度下毒性效应发生的关键基因；4）对关键基因进行功能验证，阐明盐度对BPA生物毒性复合效应产生的分子机理。

Environmental estrogens can interfere with the stability of the normal endocrine system in the body. Bisphenol A (BPA) is one of the most common environmental estrogen contaminants. It exists in large amounts in water and threatens the survival of aquatic animals such as fish. Oryzias curvinotus, commonly known as Hainan medaka, is a small-sized Chinese fish with wide salinity characteristics and is an ideal species for monitoring estrogenic toxicity in different salinity water environments. Our research team has collected the populations of the Hainan medaka in different areas of the South China Sea coast. We domesticated and maintained the Hainan medaka at different salinities in the lab. In this study, three salinity (0 ‰, 15 ‰ and 30 ‰ salinity) Hainan medaka were used as experimental subjects. The BPA generation exposure experiment was carried out on the Hainan medaka, and the toxic effect of BPA to the Hainan medaka was studied mainly as follows: .1) Determine the semi-lethal concentrations and safe concentrations of BPA at different salinities;.2) Analysis of the effects of successive generations of BPA exposure on the growth and development, gonadal differentiation and reproduction of Hainan medaka;.3) Mapping the gene and protein expression profiles of BPA exposure in successive generations of Hainan medaka, screening key genes for the toxic effect at different salinity levels.4) The functional verification of key genes was performed to elucidate the molecular mechanism of the salinity effect on the biological toxicity of BPA.