前期研究证实，杨树是三峡水库消落区生态防护林建设的适宜树种，但对其在三峡水库消落区反季节水淹后的适应机制并不清楚。杨树是雌雄异株植物，雌雄植株在应对环境胁迫时采用不同的应对策略。本项目拟以美洲黑杨的雌株丹红杨、雄株巨霸杨为研究对象，运用植物生理学及蛋白质组学的研究方法，结合亚细胞超微结构分析，模拟在三峡水库水位变动条件下，对比分析在经历不同时间的冬季水淹后，雌雄植株在不同恢复生长期的生长发育、生理生化、亚细胞超微结构、叶片全蛋白质组和叶绿体蛋白质组等方面的响应差异，从而揭示经历冬季水淹胁迫后雌雄植株在不同恢复生长期的应对策略及雌雄植株光合响应差异的分子机制。研究成果试图阐明木本植物对三峡水库消落区反季节水淹的适应机制，为消落区生态防护林建设提供理论依据与科学指导；并在分子水平上揭示雌雄异株植物在冬季水淹胁迫后的性别特异性响应机理，为木本植物抗水淹基因的筛选和克隆提供线索。

Previous studies have confirmed that poplars (Populus spp.) are ideal candidate species for the construction of ecological protection forest in the water-level-fluctuation zone in the Three Gorges Reservoir. However, adaptable mechanisms of poplars in response to reversal of flooding times in winter season is not clear. Poplars are dioecious plants. The males and females in a dioecious plant developed different strategies in response to environmental stresses. In the present study, male clones (P. deltoides CL.'Juba') and female clones (P.deltoides CL. 'Danhong'), originated from same parents, will be used as materials to test their individual repsones to winter flooding and post-flooding recovery. Sex-specific physiological, biochemical, and ultrastructural changes of both males and females at different recovery phases after different winter flooding durations will be investigated. Further molecular mechanisms involved will be elucidated based on proteomic approach, using protein samples extracted from leaf and chloroplast, respectively. The aims of this study are to compare different responsive strategies developed by males and females in P.deltoides. We mainly focus on the sex-related photosynthetic responses and molecular mechanisms between male and female in a dioecious plant after winter flooding stress. This research intends to elucidate adaptable mechanisms in woody plants in response to reversal of flooding times in winter season, and to provide certain theoretical basis and scientific instructions for the construction of ecological protection forest in the water-level-fluctuation zone in the Three Gorges Reservoir. It will further clariy the sex-specific responses in a dioecious plant to winter flooding and post flooding recovery on the molecular level, and provide some clues about screening and clone of anti-flooding genes in woody plants.