芳香烃受体（AhR）是一种配体激活的转录因子，在哺乳动物T细胞分化和免疫调控中发挥重要作用；我们前期从鱼类免疫细胞中发现了AhR具有较高的表达水平。基于此我们推测：AhR可能同样介导环境污染物引起的鱼类T细胞免疫毒性过程，并发挥重要作用。然而AhR介导的鱼类T细胞免疫毒性效应及其机制如何，目前尚无相关研究报道。本课题拟选取具有免疫毒性物质苯并[a]芘（BaP）作为AhR激动剂，利用CD4-1转基因斑马鱼分离胸腺组织作为实验材料，通过染色质免疫沉淀-测序技术在全基因组范围内鉴定T细胞中与AhR相互作用的DNA分子；同时，联合运用转录组学和蛋白质组学手段在系统水平筛选BaP胁迫下斑马鱼胸腺T细胞中重要免疫因子，构建AhR介导的免疫毒性信号通路，阐明AhR介导的BaP暴露对斑马鱼T细胞免疫毒性机理。研究结果将为深入认识环境污染物对水生生物免疫毒性效应及其机理提供重要的理论基础。

The aryl hydrocarbon receptor (AhR), a ligand-activated transcription factor that is activated by small molecules, plays an important role in T cell differentiation and immune regulation in mammals. Meanwhile, higher expression of AhR mRNA in fish immune cells was revealed in our previous study. Therefore, it is speculated that AhR may also be involved in the process of immunotoxicity induced by environmental pollutants in fish T cells, however, little is known about the mechanisms. In this project, Benzo[a]pyrene (BaP), a known immunotoxic substance, is chosen as AhR agonist, its effects are studied on transgenic zebrafish (Danio rerio) in which the CD4-1 gene is coupled with green fluorescent protein. The DNA molecules interacting with AhR in zebrafish T cells are to be identified genome-wide by chromatin immunoprecipitation-sequencing (ChIP-seq). Furthermore, this project aims to overall screen and identify the immune-associated components present in T cells using the techniques of comparative transcriptomics and proteomics. Finally, the immunomodulation mechanisms of BaP mediated by AhR on T cells in CD4-1+ transgenic zebrafish are to be elucidated. The data obtained from this project will provide a strong foundation for understanding the immunotoxic effects and mechanisms of environmental pollutants on aquatic organisms.