干旱是林木生长过程中遇到的主要非生物胁迫之一，严重影响林木的产量和质量，只有揭示林木响应干旱胁迫的信号转导机理，才能培育优质高抗林木新种质。我们前期研究发现银白杨中有三个响应干旱胁迫的调控基因：PaVAMP711-a，PaVAMP711-b和PaVAMP711-c。进一步研究表明PaVAMP711-a，PaVAMP711-b和PaVAMP711-c蛋白的亚细胞定位出现分化，并且与质膜H+-ATPase蛋白PaAHA1、PaAHA2具有相互作用。在本项目中，我们将利用分子生物学、生物化学、细胞生物学等方法，并结合银白杨转基因手段，深入解析这三个基因是如何通过影响质膜H+-ATPase活性响应干旱胁迫的分子机制，从而为培育优质高抗林木新种质提供理论支撑。

Drought is one of the main abiotic stresses encountered in the growth of forest trees, which seriously affects the yield and quality of forest trees. Revealing the signal transduction mechanism of forests in response to drought stress will help us to cultivate new high-quality and -resistant forest germplasm. Our previous study identified three regulatory genes (PaVAMP711-a, PaVAMP711-b, and PaVAMP711-c) from white poplar that respond to drought stress. Our research showed that the subcellular localization of PaVAMP711-a, PaVAMP711-b and PaVAMP711-c proteins differentiated and interacted with the plasma membrane H+-ATPase proteins PaAHA1 and PaAHA2. In this project, by using molecular biology, biochemistry, cell biology and transgenic white poplar methods, we will reveal the molecular mechanism of how these three genes respond to drought stress through plasma membrane H+-ATPase activity regulation. This study will provide theoretical support for breeding new high-quality and -resistant forest germplasm.