重复序列来源的非编码RNA在真核细胞中有着重要的功能。其中，α-satellite RNA来源于着丝粒区域，通过多种方式参与调控有丝分裂；它的表达水平异常会导致染色体分配异常。我们在前期实验中发现α-satellite RNA的表达也反过来受到细胞周期调控。同时我们发现了两种新的α-satellite RNA结合蛋白，DHX36和SAF-A；抑制它们的表达会导致类似的染色体分配异常表型。本课题拟运用多种分子和细胞生物学方法，探究驱使α-satellite RNA在细胞周期中动态转录的因素，解析α-satellite RNA与DHX36及SAF-A结合的分子细胞特性，揭示α-satellite RNA通过DHX36和SAF-A调控有丝分裂的分子机制。我们希望在此青年基金的支持下, 深入认识α-satellite RNA受到表达调控及其通过相互作用蛋白对有丝分裂进行调控的分子机理。

Repeat element-derived noncoding RNAs play important roles in eukaryotic cells. Among them, α-satellite RNA, which originates from centromeric and pericentromeric regions, was known to participate in the regulation of mitosis through various mechanisms. Abnormal expression of α-satellite RNA causes chromosome missegregation during mitosis. In our preliminary data, we observe that the expression of α-satellite RNA is dynamically regulated within the mitotic cell cycle. Meanwhile, we identify two novel α-satellite RNA binding proteins, DHX36 and SAF-A. Knocking down of these two proteins leads to similar chromosome missegregation phenotypes. In this study, we will use a variety of molecular and cellular methods to identify the factors that drive the dynamic transcription of α-satellite RNA in the mitotic cell cycle, analyze the molecular and cellular characteristics of interactions between α-satellite RNA and DHX36/SAF-A, and reveal the mechanisms that α-satellite RNA regulates mitosis through its interactions with DHX36 and SAF-A. Under the support of this grant, we hope to extensively understand the molecular mechanisms that α-satellite RNA regulates mitosis through its novel interaction partners, and discover the factors that coordinate the dynamic expression of α-satellite RNA in the cell cycle.