目前学术界普遍接受的观点认为16S rRNA既是生物学进化过程中关键的生物大分子，也是公认的原核生物进化的分子计时器。我们前期研究发现低能离子注入引发了新基因的从头起源现象，我们认为这是低能离子注入加速了原核生物分子计时器进化的结果。为了证实这一科学假说，本研究拟以细菌的多重耐药性新基因的从头起源为背景，从低能离子注入介导16S rRNA的生物学进化的视角开展研究，应用低能离子注入介导非生物合成的16S rRNA特异寡核苷酸标识序列转化细菌，构建多重耐药菌株；进而以此菌株为研究对象，综合利用基因组学、比较基因组学和分子生物学方法，阐明低能离子注入介导胞内16S rRNA基因突变与进化及其引发耐药新基因从头起源的分子机制。本研究将深化人们对低能离子注入介导生物进化过程的认识，将为低能离子注入在新基因的从头起源中的作用提供新的遗传证据，并将为超级细菌的耐药基因的形成机制研究提供新的思路。

It is generally accepted that 16S rRNA is both the key biomolecule ribosome and the molecular timer of prokaryotic microorganism. Our previous research showed that the low energy ion implantation triggered the de novo origination of new genes. This should be attributed to the result of the evolution of bacterial molecular timers mediated by low energy ion implantation. To prove this scientific hypothesis, multiple drug resistance strains (MDR) will be constructed using low energy ion implantation based on the perspective of the biological evolution of 16S rRNA. A combination genomics, comparative genomics with bioinformatics and molecular biology methods will be employed to elucidate the molecular mechanism of the mutation and evolution of 16S rRNA gene in MDR cells and the de novo origination of new drug resistance genes mediated by low energy ion implantation. This study will further help people to understand the biological evolution mediated by the low-energy ion implantation, and will also provide new evidence of the de novo origination of novel-genes mediated by the low-energy ion implantation. At the same time, this study also can help provide new research ideas for studying the MDR gene formation mechanism of the superbug strains.