转CPD基因马铃薯盐胁迫诱导的转录组和蛋白组分析

CPD基因编码C-3氧化酶，为油菜素内酯类化合物(Brassinosteroids,BRs)合成途径限速酶，BRs在植物抵抗逆境胁迫中具重要作用。但CPD基因通过调控BRs合成响应马铃薯盐胁迫的分子机理仍不清楚。前期研究表明，马铃薯CPD基因的表达水平与水分胁迫呈显著相关。基于此，本项目以前期获得的CPD基因过表达和干扰表达马铃薯为材料，进行盐胁迫处理，挖掘CPD基因的上下游调控基因和蛋白，以及响应盐胁迫的差异表达基因和蛋白，探讨这些基因和蛋白在通过调控CPD基因表达和BRs代谢网络抵抗盐胁迫中的作用；分析内源BRs含量，明确CPD基因表达水平对BRs含量的影响。同时，检测调控盐胁迫的关键转录因子及其与CPD的互作关系；筛选CPD的互作蛋白，揭示CPD通过调控马铃薯BRs合成与积累响应盐胁迫的分子机理，为马铃薯耐盐性的遗传改良提供依据。

CPD gene encodes C-3 oxidase, which plays a vital role in Brassinosteroids (BRs) biosynthesis. BRs play an important role in plant resistance to abiotic stress. However, the mechanism of CPD gene regulating the synthesis of BRs in response to salt stress in potato is still unclear. In the previous study, we found that the expression levels of CPD gene in potato were correlated with water stress. Therefore, CPD-overexpressing and interference-expressing (RNAi) transgenic potato plants will be used as experimental materials under salt stress, to excavate the upstream and downstream regulatory genes and proteins of the CPD gene, and differentially expressed genes and proteins in response to salt stress. The roles of the genes and proteins in the regulation of BRs response to salt stress will be explored. The effect of the expression level of CPD gene on the content of endogenous BRs will be also studied. And the key transcription factors regulating salt stress and CPD gene will be measured. Meanwhile, interaction between the key transcription factors and CPD gene in BRs synthetic process will be detected, and the interaction protein of CPD protein will be screened. The role of CPD in the response of potato BRs to salt sress will be analyzed, and the mechanism of CPD protein regulating the synthesis and accumulation of potato BRs will be revealed. The research will provide a theoretical basis for genetic improvement of potato salt tolerance.