Comprehensive Analysis of StSRO Gene Family and Its Expression in Response to Different Abiotic Stresses in Potato.
Comprehensive Analysis of StSRO Gene Family and Its Expression in Response to Different Abiotic Stresses in Potato.
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DOI：
10.3390/ijms232113518
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发表时间：
2022-11-04
影响因子：
5.6
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中科院分区：
文献类型：
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作者：
作为高度保守的植物特异性蛋白家族，SIMILAR-TO-RCD-ONE (SRO) 在植物生长、发育和对非生物胁迫的响应中发挥着重要作用。本研究根据马铃薯数据库DM v6.1的全基因组数据，通过搜索PARP、RST和WWE结构域，鉴定出6个StSRO基因，并根据其在染色体上的位置将其命名为StSRO1-6。采用生物信息学方法对StSRO基因进行全面分析。结果显示，6个StSRO基因不规则地分布在5条染色体上。系统发育分析表明，4个物种的30个SRO基因分布在3个类群中，而StSRO基因则分布在第II和III类群中。 StSRO基因的启动子序列含有许多与激素和应激反应相关的顺式作用元件。此外，通过在线数据库的RNA-seq数据和定量实时聚合酶链式反应（qRT-PCR）分析，分析了双单倍体（DM）马铃薯不同组织以及盐、干旱胁迫和激素处理下StSRO基因的表达水平。此外，通过转录组分析，分析了轻度、中度和重度盐胁迫下StSRO基因的表达水平。结论是StSRO基因可以响应不同的非生物条件，但其表达水平存在显着差异。本研究为进一步研究StSRO基因家族的生物学功能奠定了基础。
As a highly conserved family of plant-specific proteins, SIMILAR-TO-RCD-ONE (SROs) play an essential role in plant growth, development and response to abiotic stresses. In this study, six StSRO genes were identified by searching the PARP, RST and WWE domains based on the genome-wide data of potato database DM v6.1, and they were named StSRO1–6 according to their locations on chromosomes. StSRO genes were comprehensively analyzed using bioinformatics methods. The results showed that six StSRO genes were irregularly distributed on five chromosomes. Phylogenetic analysis showed that 30 SRO genes of four species were distributed in three groups, while StSRO genes were distributed in groups II and III. The promoter sequence of StSRO genes contained many cis-acting elements related to hormones and stress responses. In addition, the expression level of StSRO genes in different tissues of doubled monoploid (DM) potato, as well as under salt, drought stresses and hormone treatments, was analyzed by RNA-seq data from the online database and quantitative real-time polymerase chain reaction (qRT-PCR) analysis. Furthermore, the expression level of StSRO genes was analyzed by transcriptome analysis under mild, moderate and severe salt stress. It was concluded that StSRO genes could respond to different abiotic conditions, but their expression level was significantly different. This study lays a foundation for further studies on the biological functions of the StSRO gene family.
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