银屑病常见而难治，中医调治首当辨证。辨证分型也体现了银屑病的复杂多样，关于银屑病证型差异的分子机制研究还很少。前期研究表明银屑病血热证和血瘀证型存在银屑病角质形成细胞标志性蛋白—K17基因的差异表达；而诱导K17表达的细胞因子，证型间无差异。本研究提出调控血热证/血瘀证2种证型的分子机制不同，抗凋亡基因的差异表达，可以调控K17相关的信号通路的活性，影响角质形成细胞K17的表达，进而形成不同证型。本项目通过检测两证型K17相关信号通路的活性差异，并采用高通量的转录组测序技术（RNA-Seq)，筛选证型相关的抗凋亡差异表达基因，并在人HaCaT角质形成细胞中运用RNA干扰技术（RNAi）分别沉默证型相关基因，检测2种证型的细胞凋亡率、K17表达水平，以期从基因水平深入探寻银屑病血热证/血瘀证不同证型特点的分子机制，并从全新的视角认识中医证型差异的本质，为银屑病精准治疗提供更客观的证据。

Psoriasis is a very common disease and hard to cure. The different of TCM patterns is the key point of TCM treatment and the good representation of psoriatic diversity characteristics. However, there is still uncertain on mechanism of TCM patterns differentiation and need to do more research. It has been previous confirmed that there is significantly different expression of keratin 17 in both TCM patterns (heat of blood and blood stasis). However, there is no significant difference in cytokines which inducing keratin 17 expression in both TCM patterns. We proposed that it exist different mechanisms in both of TCM patterns (heat of blood and blood stasis). The psoriatic differentially expressed genes in both of psoriatic TCM patterns (heat of blood and blood stasis) may have effect on the activity of anti-apoptotic signal channels association with K17, which then result in the expression of K17 and form the different TCM patterns. In this study, we detected the activity of anti-apoptotic signal channels association with K17 in two TCM patterns. By using Whole genome RNA sequencing, we detected the differentially anti-apoptotic expressed genes in both of psoriatic TCM patterns (heat of blood and blood stasis) and selected the anti-apoptotic encoding genes association with specific TCM patterns. And then using RNA interference method to silent the specific TCM pattern associating genes so as to investigate the apoptosis rate of keratinocytes and K17 expression. We try to deeply investigate the different molecular mechanisms in both of psoriatic TCM patterns (heat of blood and blood stasis) at genetics lever. It’s new insight to deepening understanding the essence of TCM patterns differentiation and to provide the objective evidence in precision therapy of psoriasis.