运用基因芯片技术观察糖络宁对糖尿病大鼠神经细胞miRNA及相关信号通路的影响

本研究是在以往临床及实验研究的基础上，采用STZ诱导的糖尿病周围神经病变(DPN)大鼠模型，针对DPN最新底层核心发病机制微RNA及其下游信号通路，运用基因芯片技术观察糖络宁对糖尿病大鼠坐骨神经中miRNA-146a、miRNA-25、miRNA-132、miRNA-28、miRNA-200a、miRNA-144、miRNA-192,miRNA-216a /217的影响，同时观察其对大鼠坐骨神经NF-κB信号通路、PKR信号通路、MAPKs信号通路、TLRs信号通路、Nox4信号通路、FoxO3a信号通路、PI3K/Akt信号通路及Wnt/β-catenin信号通路的影响，从底层基因及分子水平阐明其作用机制，为中药治疗DPN提供科学依据，寻找中药治疗DPN的作用靶点或途径，为中药防治DPN的新药开发奠定基础。

This study is based on the early clinical and libratory research. It uses STZ to create diabetic peripheral neuropathy rat model. The study aims at miRNA- - this newest basic core mechanism of DPN and use the gene chip technology to observe the effect of Tangluoning to miRNA-146a、miRNA-25、miRNA-132、miRNA-28、miRNA-200a、miRNA-144、miRNA-192,miRNA-216a /217 of diabetic rat sciatic nerve. It also observes the effect of Tangluoning to NF-κB signaling pathway, PKR signaling pathway, MAPKs signaling pathway, TLRs signaling pathway, Nox4 signaling pathway, FoxO3a signaling pathway, PI3K/Akt signaling pathway and Wnt/β-catenin signaling pathway of diabetic rat sciatic nerve. The study wants to explore the mechanism of Tangluoning from bottom gene cell and molecular level and provide scientic evidence of Chinese Medicine treating DPN. We are looking forward the target or way of Chinese Medicine treating DPN and lay the foundation for the new treating-DPN drug exploitation.