行血益气温阳法调控Akt/GSK-3β/Snail轴抑制上皮间质转化抗卵巢癌转移的机制研究

卵巢癌发病率位居女性生殖系统恶性肿瘤第三位，但死亡率最高。肿瘤的侵袭转移是目前超过90%实体瘤患者死亡的直接原因，寻找安全有效的药物抑制肿瘤的转移是当务之急。本院肿瘤科针对卵巢癌的主要治法是行血益气温阳法，而行血益气温阳治法的核心组方是复方附苓颗粒。本课题组临床研究发现，复方附苓颗粒协同化疗药能有效抑制卵巢癌转移。近年研究表明，Akt/GSK-3β/Snail轴在抑制卵巢癌转移的关键步骤--上皮间质转化（EMT）中发挥重要作用。本课题拟通过GSK-3β敲低、GSK-3β过表达或使用Akt抑制剂后，观察复方附苓颗粒干预前后，卵巢癌细胞转移的生物学行为表现以及EMT表型、相关蛋白、转录因子、miRNA等多层次多靶点的表达情况，以期阐明以复方附苓颗粒为核心的行血益气温阳法通过调控Akt/GSK-3β/Snail轴来抑制EMT抗卵巢癌转移的分子机制。

Ovarian cancer is the third morbidity and most lethal gynecologic malignancy. Tumor invasion and metastasis is one of the more than 90% of the direct cause of death in patients with solid tumors. Looking for safe and effective pharmacological inhibition of tumor metastasis is the top priority. Our oncology department h has main therapy of promoting blood circulation tonifying qi and warming yang on ovarian cancer, which key formular is Compound Fuling Granule. Our research team found that, cooperate with chemotherapeutic drugs, Compound Fuling Granule could inhibit ovarian cancer metastasis. In recent years, studies have shown that, Akt/GSK-3β/Snail has exerted the key important effects in epithelial-mesenchymal transition to resist metastasis. This subject wants to observe the multi-level expression difference in transfer behavior,EMT phenotype, correlation protein, transcription factor,microRNA and so on, after using GSK-3β knockdown, GSK-3β overexpress,Akt inhibition and intervening with Compound Fuling Granule.We want to clarify the regulating mechanism on therapy of promoting blood circulation tonifying qi and warming yang on ovarian cancer to resist metastasis through Akt/GSK-3β/Snail axis.